Anticorps Polyclonal de lapin anti-TRAFD1

• Phare
• Validé par KD/KO

TRAFD1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 27741-1-AP

Synonymes

TRAF-type zinc finger domain-containing protein 1, Protein FLN29, FLN29

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Galerie de données de validation

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  WB analysis of HEK-293 using 27741-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27741-1-AP (TRAFD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 27741-1-AP

  WB result of TRAFD1 antibody (27741-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAFD1 transfected A549 cells.

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  WB analysis of K-562 using 27741-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 27741-1-AP (TRAFD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27741-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27741-1-AP (TRAFD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 27741-1-AP

  IP result of anti-TRAFD1 (IP:27741-1-AP, 4ug; Detection:27741-1-AP 1:800) with HEK-293 cells lysate 1575 ug.

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  IHC staining of human tonsillitis using 27741-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27741-1-AP (TRAFD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27741-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27741-1-AP (TRAFD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 27741-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27741-1-AP (TRAFD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 27741-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27741-1-AP (TRAFD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 27741-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27741-1-AP (TRAFD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 27741-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27741-1-AP (TRAFD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules HeLa, cellules K-562
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu d'amygdalite humain, human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

27741-1-AP cible TRAFD1 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRAFD1 Protéine recombinante Ag26945
• Nom complet: TRAF-type zinc finger domain containing 1
• Masse moléculaire calculée: 582 aa, 65 kDa
• Poids moléculaire observé: 65-80 kDa
• Numéro d’acquisition GenBank: BC003553
• Symbole du gène: TRAFD1
• Identification du gène (NCBI): 10906
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRAFD1, also known as TRAF-type zinc finger domain containing 1, is a protein that plays a significant role in immune system signaling pathways. It was first identified as an interferon- (IFN) and lipopolysaccharide- (LPS) inducible factor. TRAFD1 contains a TRAF-type zinc finger domain at its N-terminus and a TRAF6-binding motif in its middle region, which allows it to interact with other TRAF proteins and modulate immune responses. In the context of immune signaling, TRAFD1 has been shown to suppress the inflammatory responses to innate immunity by inhibiting Toll-like receptor 4 (TLR4) dependent NF-κB and MAPK activation in monocytes/macrophages. This suggests that TRAFD1 can act as a negative regulator of immune signaling, dampening the inflammatory response. Moreover, TRAFD1 has been implicated in human diseases. It is overexpressed in many B cell-related cancers, and single nucleotide polymorphisms (SNPs) in TRAFD1 have been linked to non-Hodgkin's lymphoma.

Publications

Species	Application	Title
human	WB	Nat Commun; Characterization of dsRNA binding proteins through solubility analysis identifies ZNF385A as a dsRNA homeostasis regulator; Authors - Na Jiang

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