Anticorps Polyclonal de lapin anti-TRAM1

TRAM1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 12705-1-AP

Synonymes

Protein TRAM1, TRAM, Translocating chain-associated membrane protein 1

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Galerie de données de validation

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  WB analysis of K-562 using 12705-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12705-1-AP (TRAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 12705-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12705-1-AP (TRAM1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12705-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12705-1-AP (TRAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12705-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12705-1-AP (TRAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse pancreas using 12705-1-AP

  IP result of anti-TRAM1 (IP:12705-1-AP, 4ug; Detection:12705-1-AP 1:1000) with mouse pancreas tissue lysate 2480 ug.

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  IHC staining of mouse skin using 12705-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12705-1-AP (TRAM1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa, cellules Jurkat, cellules PC-3
• Résultats positifs en IP: tissu pancréatique de souris,
• Résultats positifs en IHC: tissu cutané de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

12705-1-AP cible TRAM1 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRAM1 Protéine recombinante Ag3384
• Nom complet: translocation associated membrane protein 1
• Masse moléculaire calculée: 374 aa, 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC037738
• Symbole du gène: TRAM1
• Identification du gène (NCBI): 23471
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Front Pharmacol; Achyranthes bidentata Polysaccharide Activates Nuclear Factor-Kappa B and Promotes Cytokine Production in J774A.1 Cells Through TLR4/MyD88 Signaling Pathway; Authors - Sairong Fan
rat	WB	Evid Based Complement Alternat Med; The Herbal Medicine Scutellaria-Coptis Alleviates Intestinal Mucosal Barrier Damage in Diabetic Rats by Inhibiting Inflammation and Modulating the Gut Microbiota.; Authors - Boxun Zhang
human	WB	Mol Cell; An ATP13A1-assisted topogenesis pathway for folding multi-spanning membrane proteins; Authors - Jia Ji
human	WB	Front Endocrinol (Lausanne); Identification of immune-related endoplasmic reticulum stress genes in proliferative diabetic retinopathy using bioinformatics analysis; Authors - Han Chen
pig,human	WB	Vet Microbiol; Serum amyloid P component suppresses porcine epidemic diarrhea virus replication through TLR4-mediated IFN-β signaling pathway; Authors - Xinchang Lu
mouse	WB	J Inflamm Res; Circ_0001084/miR-181c-5p/PTPN4 Axis Mitigates Cardiomyocyte Injury by Modulating the TLR4/NF-κB Pathway: Insights into Therapeutic Potential for Myocardial Reperfusion Injury; Authors - Haihong Deng

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