Anticorps Polyclonal de lapin anti-transgelin/SM22-specific

transgelin/SM22-specific Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 15502-1-AP

Synonymes

SM22, SM22 alpha, SM22a, SM22alpha, Smooth muscle protein 22 alpha, TAGLN, TAGLN1, transgelin, TRANSGELIN 1

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Galerie de données de validation

• 

  WB analysis of mouse colon using 15502-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:2000 (under 10x lens).

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  IHC staining of human heart using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:2000 (under 40x lens).

• 

  IHC staining of human colon using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:2000 (under 10x lens).

• 

  IHC staining of human colon using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:2000 (under 40x lens).

• 

  IHC staining of human heart using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human kidney using 15502-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15502-1-AP (transgelin/SM22-specific antibody at dilution of 1:500 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu de côlon de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:600
• Immunohistochimie (IHC): IHC : 1:100-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

15502-1-AP cible transgelin/SM22-specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: transgelin
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22-25 kDa
• Numéro d’acquisition GenBank: NM_003186
• Symbole du gène: TAGLN
• Identification du gène (NCBI): 6876
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody is specific to transgelin 1. It does not cross-react with transgelin 2 or 3.

Publications

Species	Application	Title
mouse	WB	Physiol Genomics; Inflammation and vascular smooth muscle cell dedifferentiation following carotid artery ligation; Authors - B. Paul Herring
human	WB	Front Med (Lausanne); Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.; Authors - Fang He
human	WB	Front Genet; Bioinformatics Analysis Reveals MicroRNA-193a-3p Regulates ACTG2 to Control Phenotype Switch in Human Vascular Smooth Muscle Cells.; Authors - Weitie Wang