Anticorps Polyclonal de lapin anti-TRAP1

• Phare
• Validé par KD/KO

TRAP1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 10325-1-AP

Synonymes

HSP 75, HSP75, HSP90L, TNFR associated protein 1, TRAP 1, TRAP1

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Galerie de données de validation

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  WB analysis of HeLa using 10325-1-AP

  WB result of TRAP1 antibody (10325-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAP1 transfected HeLa cells.

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  WB analysis using 10325-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10325-1-AP (TRAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10325-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10325-1-AP (TRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10325-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10325-1-AP (TRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 10325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10325-1-AP (TRAP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 10325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10325-1-AP (TRAP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 10325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10325-1-AP (TRAP1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 10325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10325-1-AP (TRAP1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 10325-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRAP1 antibody (10325-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 10325-1-AP

  1X10^6 HeLa cells were stained with 0.2ug TRAP1 antibody (10325-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HeLa, cellules HepG2, cellules K-562, cellules NIH/3T3, cellules Raji, tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

10325-1-AP cible TRAP1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRAP1 Protéine recombinante Ag0400
• Nom complet: TNF receptor-associated protein 1
• Masse moléculaire calculée: 80 kDa
• Poids moléculaire observé: 66-70 kDa, 60 kDa
• Numéro d’acquisition GenBank: BC000406
• Symbole du gène: TRAP1
• Identification du gène (NCBI): 10131
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNF receptor-associated protein 1 (TRAP1, synonym: HSP75) is a member of the HSP90 family of molecular chaperones, and is expressed ubiquitously and located to mitochondria. A unique LxCxE motif in HSP75, but not in other HSP90 family members, appears to be important for binding to the simian virus 40 T-antigen-binding domain of hypophosphorylated Rb. Data also suggests that suppression of the expression of TRAP1 in mitochondria plays an important role in the induction of apoptosis caused via formation of reactive oxygen species (ROS).

Publications

Species	Application	Title
human	WB, IF	J Cell Biol; Parkin-independent mitophagy via Drp1-mediated outer membrane severing and inner membrane ubiquitination.; Authors - Yumiko Oshima
mouse	WB,IF	Glia; The LncRNA AK018453 regulates TRAP1/Smad signaling in IL-17-activated astrocytes: A potential role in EAE pathogenesis.; Authors - Qingxiu Zhang ; KD Validated
mouse	WB	J Gerontol A Biol Sci Med Sci; Effects of Heat Stress Treatment on Age-dependent Unfolded Protein Response in Different Types of Skeletal Muscle.; Authors - Yuki Tamura
human	IHC	Sci Rep; Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.; Authors - Katsutaka Mitachi
mouse	IF	Cell Signal; Downregulated fat mass and obesity-associated protein inhibits bone resorption and osteoclastogenesis by nuclear factor-kappa B inactivation; Authors - Jinpeng Zhuang
mouse	WB	IUBMB Life; Loss of high-temperature requirement protein A2 protease activity induces mitonuclear imbalance via differential regulation of mitochondrial biogenesis in sarcopenia.; Authors - Haohan Zhou