Anticorps Polyclonal de lapin anti-TREX1

• Phare
• Validé par KD/KO

TREX1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

Avis (1)

N° de cat : 24876-1-AP

Synonymes

DRN3, Deoxyribonuclease III, CRV, AGS5, 3'-5' exonuclease TREX1

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Galerie de données de validation

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  WB analysis using 24876-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24876-1-AP (TREX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24876-1-AP

  IP result of anti-TREX1 (IP:24876-1-AP, 4ug; Detection:24876-1-AP 1:300) with HeLa cells lysate 1200ug.

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  IF Staining of HeLa using 24876-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TREX1 antibody (24876-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

24876-1-AP cible TREX1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: bovin, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TREX1 Protéine recombinante Ag18325
• Nom complet: three prime repair exonuclease 1
• Masse moléculaire calculée: 369 aa, 39 kDa
• Poids moléculaire observé: 32-39 kDa
• Numéro d’acquisition GenBank: BC023630
• Symbole du gène: TREX1
• Identification du gène (NCBI): 11277
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TREX1 (three prime repair exonuclease 1), also known as trophoblast expressed 1, CRV, AGS1, AGS5, DRN3, HERNS or DNase III, is a member of the exonuclease superfamily and belongs to the TREX family. TREX1 may play a role in DNA repair. TREX1 is expressed in thymus, spleen, liver, brain, heart, small intestine and colon. Mutations or defects in the gene encoding TREX1 have been associated with a variety of diseases, including systemic lupus erythematosus, chilblain lupus (CHBL), Aicardi-Goutieres syndrome type 1 (AGS1) and type 5 (AGS5).

Publications

Species	Application	Title
		J Neuropathol Exp Neurol; Retinal Vasculopathy With Cerebral Leukodystrophy: Clinicopathologic Features of an Autopsied Patient With a Heterozygous TREX 1 Mutation.; Authors - Rie Saito
human	WB	bioRxiv; Pathogen-driven CRISPR screens identify TREX1 as a regulator of DNA self-sensing during influenza virus infection; Authors - Cason R King ; KO Validated
human	IF,WB	Cell Host Microbe; Pathogen-driven CRISPR screens identify TREX1 as a regulator of DNA self-sensing during influenza virus infection; Authors - Cason R King
human	WB	Cell Rep; Pharmacological inhibition of neddylation impairs long interspersed element 1 retrotransposition; Authors - Yan Li
bovine	WB	Int J Mol Sci; Inactivation of Myostatin Delays Senescence via TREX1-SASP in Bovine Skeletal Muscle Cells; Authors - Miaomiao Yang
human	WB	Front Immunol; Two rare mutations in homozygosity synergize to silence TREX1 in Aicardi-Goutières syndrome; Authors - Tamar Rubin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chantal (Verified Customer) (10-04-2022)

reconignises the specific band, validated by KO of TREX1. A little bit of background. the signal is weak at 1:1000 dilution, better at 1:500. Works on human and mouse cell lines.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: MEFs, Hela, HEK293FT, RPE1

TREX1 Antibody Western Blot validation (1:500 dilution) in MEFs, Hela, HEK293FT, RPE1 (Cat no:24876-1-AP)

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