Anticorps Polyclonal de lapin anti-TRIB2

• Phare
• Validé par KD/KO

TRIB2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 15359-1-AP

Synonymes

C5FW, GS3955, TRB 2, TRB2, TRIB2, Tribbles homolog 2

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Galerie de données de validation

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  WB analysis of HeLa using 15359-1-AP

  WB result of TRIB2 antibody (15359-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIB2 transfected HeLa cells.

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  WB analysis of HeLa using 15359-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15359-1-AP (TRIB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 15359-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15359-1-AP (TRIB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15359-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15359-1-AP (TRIB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15359-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15359-1-AP (TRIB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules K-562, tissu de thymus de souris
• Résultats positifs en IHC: tissu placentaire humain, tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu hépatique humain, tissu ovarien humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

15359-1-AP cible TRIB2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIB2 Protéine recombinante Ag7605
• Nom complet: tribbles homolog 2 (Drosophila)
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC002637
• Symbole du gène: TRIB2
• Identification du gène (NCBI): 28951
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIB2, also named as TRB2 , belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and Tribbles subfamily. TRIB2 interacts with MAPK kinases and regulates activation of MAP kinases. It does not display kinase activity. Antibodies against TRIB2 are present in sera from patients with autoimmune uveitis(uniprot). TRIB2 is enriched in hypocretin neurons. Sera from narcolepsy patients with cataplexy had higher Trib2-specific antibody titers compared with either normal controls or patients with idiopathic hypersomnia, multiple sclerosis, or other inflammatory neurological disorders.

Publications

Species	Application	Title
mouse,human	WB,IF	iScience; FUS-dependent microRNA deregulations identify TRIB2 as a druggable target for ALS motor neurons; Authors - Wan Yun Ho
mouse	WB,IF	Exp Mol Med; Trib2 regulates the pluripotency of embryonic stem cells and enhances reprogramming efficiency.; Authors - Eun Kyoung Do ; KD Validated