Anticorps Polyclonal de lapin anti-TRIM14

• Phare
• Validé par KD/KO

TRIM14 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(15)

N° de cat : 15742-1-AP

Synonymes

Tripartite motif-containing protein 14, tripartite motif containing 14, TRIM, KIAA0129

---

Galerie de données de validation

• 

  WB analysis using 15742-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15742-1-AP (TRIM14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 15742-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15742-1-AP (TRIM14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 15742-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 15742-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 15742-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 15742-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 15742-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TRIM14 antibody (15742-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules L02, cellules HepG2, cellules HL-60, cellules Jurkat
• Résultats positifs en IHC: tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

15742-1-AP cible TRIM14 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM14 Protéine recombinante Ag8158
• Nom complet: tripartite motif-containing 14
• Masse moléculaire calculée: 442 aa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC006333
• Symbole du gène: TRIM14
• Identification du gène (NCBI): 9830
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tripartite motif-containing 14(TRIM14) belongs to the TRIM protein family that contains 3 zinc-binding domains, a RING, a B-box type1 and a B-box type2 and plays key roles in cellular proliferation, differentiation, development, transformation, and immune response mechanisms . TRIM4 inhibits the transcriptional activity of SPI1 in a dose-dependent manner. It may participate in the early stages of differentiation of embryonic stem cells, leading to the formation of embryoid bodies.

Publications

Species	Application	Title
human,mouse	WB,IF,IP	Cell Rep; Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells; Authors - Yingqi Liu ; KO Validated
human	WB	Sci Rep; TRIM14 is a Putative Tumor Suppressor and Regulator of Innate Immune Response in Non-Small Cell Lung Cancer.; Authors - Josephine Hai ; KD Validated
human	WB	Sci Rep; TRIM14 regulates cell proliferation and invasion in osteosarcoma via promotion of the AKT signaling pathway.; Authors - Guoxing Xu ; KD Validated
human	WB	Sci Rep; Expression profiling of TRIM protein family in THP1-derived macrophages following TLR stimulation.; Authors - Mei-Xiu Jiang
human	WB,IHC,IF	Carcinogenesis; TRIM14 suppressed the progression of NSCLC via hexosamine biosynthesis pathway; Authors - Sisi Wei
human	WB	Front Pharmacol; Hypoxia-Induced Upregulation of lncRNA ELFN1-AS1 Promotes Colon Cancer Growth and Metastasis Through Targeting TRIM14 via Sponging miR-191-5p.; Authors - Xu Jing

{{ptg:RelatedPrimaryAntibodies}}