Anticorps Polyclonal de lapin anti-TRIM15

• Phare
• Validé par KD/KO

TRIM15 Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 13623-1-AP

Synonymes

RING finger protein 93, RNF93, TRIM15, tripartite motif containing 15, Zinc finger protein 178, Zinc finger protein B7, ZNF178, ZNFB7

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Galerie de données de validation

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  WB analysis of Jurkat using 13623-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13623-1-AP (TRIM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13623-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13623-1-AP (TRIM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

13623-1-AP cible TRIM15 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM15 Protéine recombinante Ag4533
• Nom complet: tripartite motif-containing 15
• Masse moléculaire calculée: 465 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC038585
• Symbole du gène: TRIM15
• Identification du gène (NCBI): 89870
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM15 (Tripartite motif-containing protein 15) is also named as RNF93, ZNF178, ZNFB7 and belongs to the TRIM/RBCC family. It acts as an ubiquitin-protein ligase and contributes to the endogenous restriction of retroviruses in cells.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; TRIM15 and CYLD regulate ERK activation via lysine-63-linked polyubiquitination.; Authors - Guixin Zhu ; KO Validated
human	IF	J Cell Sci; TRIM15 is a focal adhesion protein that regulates focal adhesion disassembly.; Authors - Pradeep D Uchil ; KD Validated
human	IHC	iScience; Whole-genome DNA methylation and DNA methylation-based biomarkers in lung squamous cell carcinoma; Authors - Qidong Cai
human	WB	J Virol; TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity.; Authors - Uchil Pradeep D PD ; KD Validated
human	IHC	J Cancer; Tripartite motif-containing 15 overexpression in non-small cell lung cancer is associated with poor patient prognoses.; Authors - Xiaoying Han
human	WB	Cell Death Dis; TRIM15 forms a regulatory loop with the AKT/FOXO1 axis and LASP1 to modulate the sensitivity of HCC cells to TKIs; Authors - Chong Yang