Anticorps Polyclonal de lapin anti-TRIM21

• Phare
• Validé par KD/KO

TRIM21 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (4)

Applications

WB, IHC, IF/ICC, IP, coIP, ELISA

Conjugaison

Non conjugué

Publications(88)

Avis (5)

N° de cat : 12108-1-AP

Synonymes

52 kDa ribonucleoprotein autoantigen Ro/SS-A, E3 ubiquitin-protein ligase TRIM21, EC:2.3.2.27, RING finger protein 81, RNF 81

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Galerie de données de validation

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  WB analysis using 12108-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12108-1-AP

  WB result of TRIM21 antibody (12108-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM21 transfected HeLa cells.

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  WB analysis using 12108-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 12108-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12108-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 12108-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12108-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12108-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HT-29 using 12108-1-AP

  IP result of anti-TRIM21 (IP:12108-1-AP, 4ug; Detection:12108-1-AP 1:5000) with HT-29 cells lysate 1760 ug.

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  IHC staining of human pancreas cancer using 12108-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12108-1-AP (TRIM21 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12108-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12108-1-AP (TRIM21 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12108-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12108-1-AP (TRIM21 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12108-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12108-1-AP (TRIM21 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 12108-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRIM21 antibody (12108-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 12108-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRIM21 antibody (12108-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 12108-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TRIM21 antibody (12108-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules HeLa, cellules HL-60, cellules HT-1080, cellules HT-29, cellules MCF-7
• Résultats positifs en IP: cellules HT-29,
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 29 publications below
• WB: See 75 publications below
• IHC: See 24 publications below
• IF: See 27 publications below
• IP: See 20 publications below
• CoIP: See 14 publications below

Informations sur le produit

12108-1-AP cible TRIM21 dans les applications de WB, IHC, IF/ICC, IP, coIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, porc, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM21 Protéine recombinante Ag2753
• Nom complet: tripartite motif-containing 21
• Masse moléculaire calculée: 475 aa, 54 kDa
• Poids moléculaire observé: 42-45 kDa, 49-54 kDa
• Numéro d’acquisition GenBank: BC010861
• Symbole du gène: TRIM21
• Identification du gène (NCBI): 6737
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM21 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle , which composed of a single polypeptide and 1 of 4 small RNA molecules. TRIM21 exists various isoforms and range molecular weight of isoforms is about 42-45kDa and 49-54 kDa.

Publications

Species	Application	Title
mouse	WB	Blood; Platelet NLRP6 protects against microvascular thrombosis in sepsis; Authors - Huimin Jiang
human	IHC	J Clin Invest; The ubiquitin ligase TRIM21 regulates mutant p53 accumulation and gain-of-function in cancer; Authors - Juan Liu
human	WB,IP	Adv Sci (Weinh); SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma; Authors - Qifan Hu
human	WB	Autophagy; Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules; Authors - Cuiwei Yang ; KD Validated
human	WB,IP	Nat Commun; Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.; Authors - Jiawei Zhang
human	WB	Dev Cell; Lactylation of LSD1 is an acquired epigenetic vulnerability of BRAFi/MEKi-resistant melanoma; Authors - Aicun Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Madison (Verified Customer) (05-28-2024)

The western blot showed very clear bands and had really precise staining. We got exactly what we wanted from the antibody on the first try

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Rippa Insoluble treated mouse cell

TRIM21 Antibody Western Blot validation (1:500 dilution) in Rippa Insoluble treated mouse cell (Cat no:12108-1-AP)

FH

Paul (Verified Customer) (01-14-2020)

Used routinely for Western Blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEKs

FH

Laura (Verified Customer) (01-14-2020)

Gives a clean Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293T

FH

Benjamin (Verified Customer) (01-10-2020)

Used many times in many WB and IF applications. Reliably antibody which detects TRIM21 basal as well as fusion proteins in WB and can be used to visualise TRIM21 in cells through IF.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:500 WB, 1:200 IF
• Cell Tissue Type: HEK, SHSY5Y

FH

Alinda (Verified Customer) (09-11-2019)

HEK were transfected with a TRIM21 overexpression vector- secondary anitbody used was rb Alexa 488.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: HEK-293T

TRIM21 Antibody Immunofluorescence, validation (1:200 dilution) in HEK-293T (Cat no:12108-1-AP)

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