Anticorps Polyclonal de lapin anti-TRIM24

• Phare
• Validé par KD/KO

TRIM24 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(46)

Avis (1)

N° de cat : 14208-1-AP

Synonymes

TIF1-alpha, TIF1A, TIF1, RING-type E3 ubiquitin transferase TIF1-alpha, EC:2.3.2.27

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Galerie de données de validation

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  WB analysis of HeLa using 14208-1-AP

  WB result of TRIM24 antibody (14208-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM24 transfected HeLa cells.

• 

  WB analysis using 14208-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14208-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BGC-823 using 14208-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14208-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 Antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

• 

  WB analysis of L02 using 14208-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14208-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14208-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14208-1-AP

  IP result of anti-TRIM24 (IP:14208-1-AP, 4ug; Detection:14208-1-AP 1:6000) with HeLa cells lysate 1085 ug.

• 

  IHC staining of human prostate cancer using 14208-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14208-1-AP (TRIM24 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human prostate cancer using 14208-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14208-1-AP (TRIM24 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 14208-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRIM24 antibody (14208-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules BGC-823, cellules L02, cellules MCF-7, tissu cardiaque humain, tissu de muscle squelettique de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 13 publications below
• WB: See 35 publications below
• IHC: See 18 publications below
• IF: See 8 publications below
• IP: See 5 publications below
• CoIP: See 1 publications below
• ChIP: See 5 publications below
• RIP: See 1 publications below

Informations sur le produit

14208-1-AP cible TRIM24 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM24 Protéine recombinante Ag5417
• Nom complet: tripartite motif-containing 24
• Masse moléculaire calculée: 117 kDa
• Poids moléculaire observé: 117 kDa
• Numéro d’acquisition GenBank: BC028689
• Symbole du gène: TRIM24
• Identification du gène (NCBI): 8805
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM24, also named as RNF82, TIF1 and TIF1A, interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors, it requires the presence of estradiol. It is a regulation protein of P53. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) wich is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue.

Publications

Species	Application	Title
human	IHC	Nature; TRIM24 links a non-canonical histone signature to breast cancer.; Authors - Tsai Wen-Wei WW
human	WB,CoIP	Mol Cell; p53 engages the cGAS/STING cytosolic DNA sensing pathway for tumor suppression; Authors - Monisankar Ghosh ; KD Validated
human	WB	Mol Cell; TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains.; Authors - Ai Kaiho-Soma
human	WB	Nat Chem Biol; Functional TRIM24 degrader via conjugation of ineffectual bromodomain and VHL ligands.; Authors - Lara N Gechijian
human	WB, IP, IHC	Nat Commun; TRIM24 is an oncogenic transcriptional co-activator of STAT3 in glioblastoma.; Authors - Deguan Lv
human	WB,IF	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Parijat (Verified Customer) (09-20-2023)

Good antibody for Western

• Applications: Western Blot

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