Anticorps Polyclonal de lapin anti-TRIM29

• Phare
• Validé par KD/KO

TRIM29 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(13)

N° de cat : 17542-1-AP

Synonymes

Ataxia telangiectasia group D-associated protein, ATDC, tripartite motif containing 29, Tripartite motif-containing protein 29

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Galerie de données de validation

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  WB analysis using 17542-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of BGC-823 using 17542-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 17542-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 17542-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 17542-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 17542-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17542-1-AP (TRIM29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 17542-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17542-1-AP (TRIM29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 17542-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 17542-1-AP (TRIM29 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 17542-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17542-1-AP (TRIM29 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 17542-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17542-1-AP (TRIM29 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 17542-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 17542-1-AP (TRIM29 Antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules A431, cellules BGC-823
• Résultats positifs en IHC: tissu de cancer de la peau humain, tissu cutané humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:20000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

17542-1-AP cible TRIM29 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM29 Protéine recombinante Ag11600
• Nom complet: tripartite motif-containing 29
• Masse moléculaire calculée: 588 aa, 66 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC017352
• Symbole du gène: TRIM29
• Identification du gène (NCBI): 23650
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM29(Tripartite motif-containing protein 29) is also named as ATDC( Ataxia telangiectasia group D-associated protein). ATDC protein physically interacted with the intermediate filament protein vimentin, a protein kinase C substrate and colocalizing protein, and with an inhibitor of protein kinase C, PKCI1 (PMID:7644499). Knockdown of TRIM29 in airway epithelial cells enhances type I interferon production, and in human nasopharyngeal carcinoma cells results in almost complete Epstein-Barr virus clearance. TRIM29 is also highly induced by cytosolic double-stranded DNA in myeloid dendritic cells (PMID: 29038422). Western detected both full-length TRIM29 (65 kDa) and a truncated variant of TRIM29 with a molecular weight of about 50 kDa (PMID: 26095369).

Publications

Species	Application	Title
mouse,human	WB,IP,IHC	Mol Cancer; CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer.; Authors - Tao Jiang ; KD Validated
human	WB	Oncogenesis; Galectin-9 promotes a suppressive microenvironment in human cancer by enhancing STING degradation.; Authors - Chuan-Xia Zhang
		iScience; Alternative transcribed 3' isoform of long non-coding RNA Malat1 inhibits mouse retinal oxidative stress; Authors - Amr R Ghanam
human	WB,IHC	PLoS One; Ataxia-Telangiectasia Group D Complementing Gene (ATDC) Promotes Lung Cancer Cell Proliferation by Activating NF-κB Pathway.; Authors - Tang Zhong-Ping ZP
		Cancer Sci; Role of deltaNp63(pos) CD44v(pos) cells in the development of N-nitroso-tris-chloroethylurea-induced peripheral-type mouse lung squamous cell carcinomas.; Authors - Shotaro Yamano
human	WB	Cancer Biol Ther; iTRAQ-based quantitative proteomic analysis of differentially expressed proteins in chemoresistant nasopharyngeal carcinoma.; Authors - Kun Wang

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