Anticorps Polyclonal de lapin anti-TRIM31

• Phare
• Validé par KD/KO

TRIM31 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 12543-1-AP

Synonymes

C6orf13, HCG1, HCGI, RNF, TRIM31, tripartite motif containing 31

---

Galerie de données de validation

• 

  WB analysis of COLO 320 using 12543-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12543-1-AP (TRIM31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HT-29 using 12543-1-AP

  IP result of anti-TRIM31(IP:12543-1-AP, 4ug; Detection:12543-1-AP 1:2000) with HT-29 cells lysate 1040 ug.

• 

  IHC staining of human small intestine using 12543-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12543-1-AP (TRIM31 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 12543-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12543-1-AP (TRIM31 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules COLO 320
• Résultats positifs en IP: cellules HT-29,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

12543-1-AP cible TRIM31 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM31 Protéine recombinante Ag3264
• Nom complet: tripartite motif-containing 31
• Masse moléculaire calculée: 425 aa, 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC017017
• Symbole du gène: TRIM31
• Identification du gène (NCBI): 11074
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM31(Tripartite motif-containing protein 31) is also named as C6orf13, RNF and belongs to the TRIM/RBCC family. The deduced protein contains an N-terminal RING domain, a B-box-2 domain, and a coiled-coil region. TRIM31 forms homodimers(PMID:11331580). This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB,IF	Mol Cell; Arginine monomethylation by PRMT7 controls MAVS-mediated antiviral innate immunity.; Authors - Junji Zhu ; KO Validated
human	WB	Nat Commun; USP18 positively regulates innate antiviral immunity by promoting K63-linked polyubiquitination of MAVS.; Authors - Jinxiu Hou
human	WB	Nat Commun; TRIM31 promotes Atg5/Atg7-independent autophagy in intestinal cells.; Authors - Eun A Ra
mouse,rat	WB,IF	Redox Biol; The E3 ubiquitin ligase TRIM31 is involved in cerebral ischemic injury by promoting degradation of TIGAR.; Authors - Shenglan Zeng ; KD Validated
human	WB	J Virol; TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity.; Authors - Uchil Pradeep D PD
mouse	WB	J Immunol; AKT Regulates NLRP3 Inflammasome Activation by Phosphorylating NLRP3 Serine 5.; Authors - Wei Zhao