Anticorps Polyclonal de lapin anti-TRIM32

• Phare
• Validé par KD/KO

TRIM32 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(15)

Avis (1)

N° de cat : 10326-1-AP

Synonymes

72 kDa Tat-interacting protein, E3 ubiquitin-protein ligase TRIM32, EC:2.3.2.27, HT2A, RING-type E3 ubiquitin transferase TRIM32

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Galerie de données de validation

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  WB analysis using 10326-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10326-1-AP (TRIM32 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 10326-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10326-1-AP (TRIM32 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10326-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10326-1-AP (TRIM32 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of U-251 using 10326-1-AP

  IP result of anti-TRIM32 (IP:10326-1-AP, 4ug; Detection:10326-1-AP 1:2000) with U-251 cells lysate 1200 ug.

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  IHC staining of human colon using 10326-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10326-1-AP (TRIM32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10326-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10326-1-AP (TRIM32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10326-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10326-1-AP (TRIM32 antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human colon using 10326-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10326-1-AP (TRIM32 antibody) at dilution of 1:25 (under 40x lens).

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  IF Staining of mouse embryo using 10326-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using TRIM32 antibody (10326-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of hTERT-RPE1 cells using 10326-1-AP

  IF result (cytoplasm stain) of anti-BBS11 (10326-1-AP; 1:50) with hTERT-RPE1 cell by Dr. Moshe Kim.

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  IF Staining of hTERT-RPE1 using 10326-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using TRIM32 antibody (10326-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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  FC experiment of A431 using 10326-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human TRIM32 (10326-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules NIH/3T3, tissu de côlon de souris
• Résultats positifs en IP: cellules U-251,
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu embryonnaire de souris,
• Résultats positifs en IF/ICC: cellules hTERT-RPE1
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:100-1:400
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 12 publications below
• IHC: See 4 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

10326-1-AP cible TRIM32 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM32 Protéine recombinante Ag0401
• Nom complet: tripartite motif-containing 32
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 70-72 kDa
• Numéro d’acquisition GenBank: BC003154
• Symbole du gène: TRIM32
• Identification du gène (NCBI): 22954
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM32(Tripartite motif-containing protein 32) is a widely expressed ubiquitin ligase that is localized to the Z-line in skeletal muscle(PMID:19349376). It promotes PIAS4 ubiquitination and degradation, and by controlling PIAS4 stability, regulates UVB-induced keratinocyte apoptosis through induction of NFkappaB(PMID: 16816390). Western blot analysis detected TRIM32 at an apparent molecular mass of 70-72 kDa.

Publications

Species	Application	Title
human	WB	Sci Adv; An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis.; Authors - Molly M Wilson
mouse,human	IHC	J Am Soc Nephrol; Proteome Analysis of Isolated Podocytes Reveals Stress Responses in Glomerular Sclerosis.; Authors - Sybille Koehler
mouse	WB	Hum Mol Genet; Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component.; Authors - Kudryashova Elena E ; KO Validated
human	WB	PLoS Pathog; TRIM32 inhibits Venezuelan equine encephalitis virus infection by targeting a late step in viral entry; Authors - Yifan Xie ; KD Validated
rat,human	WB,IP,IF	FASEB J; Ubiquitin ligase TRIM32 promotes dendrite arborization by mediating degradation of the epigenetic factor CDYL.; Authors - Lei Liu ; KD Validated
human	IF	J Cell Sci; TRIM32, but not its muscular dystrophy-associated mutant, positively regulates and is targeted to autophagic degradation by p62/SQSTM1.; Authors - Katrine Stange Overå

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ashraf (Verified Customer) (08-22-2024)

We have so far struggled with detecting the knockdown/knockout of Trim32 in our experimental settings despite testing several antibodies. We could very clearly see downregulation or absence of Trim32 as expected at transcript level but failed to detect the same at protein level. However, use of Trim32 Ab from Proteintech has unbelievably solved this issue! Highly highly recommended *****

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: heart, liver, bat

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