Anticorps Recombinant de lapin anti-TRIM46

TRIM46 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240557C2

N° de cat : 83614-5-RR

Synonymes

Tripartite, fibronectin type-III and C-terminal SPRY motif protein, Tripartite motif-containing protein 46, tripartite motif containing 46, TRIFIC, GeneY

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Galerie de données de validation

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  IF Staining of HepG2 using 83614-5-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TRIM46 antibody (83614-5-RR, Clone: 240557C2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  FC experiment of HeLa using 83614-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.2 ug TRIM46 Recombinant antibody (83614-5-RR, Clone:240557C2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83614-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83614-5-RR against Human TRIM46 were performed. The affinity constant is 1.48 nM.

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83614-5-RR cible TRIM46 dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TRIM46 Protéine recombinante Ag15290
• Nom complet: tripartite motif-containing 46
• Masse moléculaire calculée: 759 aa, 83 kDa
• Numéro d’acquisition GenBank: BC069416
• Symbole du gène: TRIM46
• Identification du gène (NCBI): 80128
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tripartite motif-containing protein 46 (TRIM46), a microtubule-associated protein, is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon specification, the establishment of neuronal polarity, and proper neuronal migration. The TRIM46 protein reported in previous studies includes the long isoform TRIM46L (83 kDa) and the short isoform TRIM46S (60 kDa).

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