Anticorps Recombinant de lapin anti-TRIM62

TRIM62 Recombinant Antibody for IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

230195G5

N° de cat : 82892-1-RR

Synonymes

230195G5, E3 ubiquitin-protein ligase TRIM62, EC:2.3.2.27, tripartite motif containing 62, Tripartite motif-containing protein 62

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Galerie de données de validation

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  IF Staining of U2OS using 82892-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TRIM62 antibody (82892-1-RR, Clone: 230195G5 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 82892-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human TRIM62 (82892-1-RR, Clone:230195G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.5 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82892-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82892-1-RR against Human TRIM62 were performed. The affinity constant is 90.8 pM.

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Applications testées

• Résultats positifs en IF/ICC: cellules U2OS,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82892-1-RR cible TRIM62 dans les applications de IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TRIM62 Protéine recombinante Ag7143
• Nom complet: tripartite motif-containing 62
• Masse moléculaire calculée: 54 kDa
• Numéro d’acquisition GenBank: BC001222
• Symbole du gène: TRIM62
• Identification du gène (NCBI): 55223
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM62(Tripartite motif-containing protein 62) belongs to the TRIM/RBCC family. TRIM proteins are involved in a broad range of biological processes, including some that underlie genetic disorders, neurological disorders and cancers. TRIM62 is shown to promote the release of HIV from infected cells, and a TRIM62 protein that has a mutation in its RING domain was more active than the native protein in promoting HIV release(PMID:18836477).

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