Anticorps Polyclonal de lapin anti-TRMT6

• Phare
• Validé par KD/KO

TRMT6 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

Avis (1)

N° de cat : 16727-1-AP

Synonymes

tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6, TRMT 6, TRM6, mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6, CGI-09

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Galerie de données de validation

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  WB analysis using 16727-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16727-1-AP (TRMT6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16727-1-AP

  WB result of TRMT6 antibody (16727-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRMT6 transfected HepG2 cells.

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  IP experiment of HeLa using 16727-1-AP

  IP result of anti-TRMT6 (IP:16727-1-AP, 4ug; Detection:16727-1-AP 1:700) with HeLa cells lysate 2000 ug.

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  IHC staining of human colon cancer using 16727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16727-1-AP (TRMT6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16727-1-AP (TRMT6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HuH-7, cellules SMMC-7721
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Informations sur le produit

16727-1-AP cible TRMT6 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRMT6 Protéine recombinante Ag10176
• Nom complet: tRNA methyltransferase 6 homolog (S. cerevisiae)
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC001262
• Symbole du gène: TRMT6
• Identification du gène (NCBI): 51605
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transfer RNA (tRNA) modifications help regulate the efficiency of mRNA translation by maintaining the correct reading frames within mRNA. TRMT6 (tRNA methyltransferase 6 ), also known as GCD10 or TRM6, is a 497 amino acid protein that localizes to the nucleus and exists as a heterodimer with TRM61. Expressed in liver, brain, ovary and testis, TRMT6 functions as a substrate-binding subunit of tRNA and is thought to catalyze the formation of N(1)-methyladenine at position 58 in initiator methionyl-tRNA. TRMT6 exists as three alternatively spliced isoforms that, in response to DNA damage, may be phosphorylated by ATM or ATR. This antibody is specific to 56kd TRMT6 protein.

Publications

Species	Application	Title
mouse	WB	Acta Pharm Sin B; ADAR1 regulates vascular remodeling in hypoxic pulmonary hypertension through N1-methyladenosine modification of circCDK17; Authors - Junting Zhang
mouse	WB, IHC	Nat Commun; N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.; Authors - Yanying Wang
human	WB	Cell Discov; ALKBH3-dependent m1A demethylation of Aurora A mRNA inhibits ciliogenesis.; Authors - Wenjun Kuang
mouse	WB	Nat Immunol; tRNA-m1A modification promotes T cell expansion via efficient MYC protein synthesis; Authors - Yongbo Liu ; KO Validated
human	WB	Cell Death Discov; N1-Methyladenosine modification of mRNA regulates neuronal gene expression and oxygen glucose deprivation/reoxygenation induction; Authors - Zhangyang Qi
human	WB	Cancer Lett; YTHDF3 Drives Tumor Growth and Metastasis by Recruiting eIF4B to Promote Notch2 Translation in Breast Cancer; Authors - Hongyu Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Estefania (Verified Customer) (05-16-2022)

Very good signal. Tested using simple western (ProteinSimple)

• Applications: Western Blot
• Primary Antibody Dilution: 1:20
• Cell Tissue Type: HepG2 cell lysate

TRMT6 Antibody Western Blot validation (1:20 dilution) in HepG2 cell lysate (Cat no:16727-1-AP)

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