Anticorps Polyclonal de lapin anti-TRPV6

TRPV6 Polyclonal Antibody for IF, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 13411-1-AP

Synonymes

ABP/ZF, Calcium transport protein 1, CaT L, CaT like, CAT1, CATL, ECAC2, Epithelial calcium channel 2, HSA277909, LP6728, TRPV6, ZFAB

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Galerie de données de validation

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  WB analysis of human placenta using 13411-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13411-1-AP (TRPV6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 13411-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13411-1-AP (TRPV6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 13411-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13411-1-AP (TRPV6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 13411-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13411-1-AP (TRPV6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 13411-1-AP

  IP Result of anti-TRPV6 (IP:13411-1-AP, 4ug; Detection:13411-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

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  IF Staining of HeLa using 13411-1-AP

  Immunofluorescent analysis of Hela cells, using TRPV6 antibody 13411-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules PC-3, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IF: See 4 publications below

Informations sur le produit

13411-1-AP cible TRPV6 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRPV6 Protéine recombinante Ag4060
• Nom complet: transient receptor potential cation channel, subfamily V, member 6
• Masse moléculaire calculée: 725 aa, 83 kDa
• Poids moléculaire observé: 83 kDa, 70 kDa
• Numéro d’acquisition GenBank: BC034814
• Symbole du gène: TRPV6
• Identification du gène (NCBI): 55503
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRPV6, also named as CAT1 or ECaC2, is a member of the transient receptor potential (TRP) family of membrane proteins. Unlike most TRP channels, TRPV6 and its closest relative, TRPV5, are calcium-selective channels. TRPV6 is highly expressed in the proximal intestine, placenta and exocrine tissues (PMID: 12869611). It is probably involved in calcium absorption in various tissues, including calcium reabsorption in kidney. TRPV6 is overexpressed in some cancers and exhibits oncogenic potential.

Publications

Species	Application	Title
human	WB	J Mol Cell Cardiol; TRPV6 protects ER stress-induced apoptosis via ATF6α-TRPV6-JNK pathway in human embryonic stem cell-derived cardiomyocytes.; Authors - Zhichao Li
human	IF	iScience; A microengineered Brain-Chip to model neuroinflammation in humans; Authors - Iosif Pediaditakis
human	WB,IF	Mol Cancer Res; PMID: 19996302; Authors - Bolanz Katrin A KA
mouse	WB	Front Pharmacol; Aqueous Extract of Mori Folium Exerts Bone Protective Effect Through Regulation of Calcium and Redox Homeostasis via PTH/VDR/CaBP and AGEs/RAGE/Nox4/NF-κB Signaling in Diabetic Rats.; Authors - Chenyue Liu
human	WB	J Gastroenterol Hepatol; Vitamin D receptor is overexpressed in the duodenum of patients with irritable bowel syndrome.; Authors - Ko Miura
mouse	WB	Mol Reprod Dev; Proteomic analysis of seminal extracellular vesicle proteins involved in asthenozoospermia by iTRAQ.; Authors - Yu Lin

Produits associés

TRPV1 Polyclonal antibody

22686-1-AP

TRPV4 Polyclonal antibody

20987-1-AP

TRPV5 Polyclonal antibody

17322-1-AP