Anticorps Polyclonal de lapin anti-TSC22D2

TSC22D2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 25418-1-AP

Synonymes

TSC22 domain family protein 2, TILZ4c, TILZ4b, TILZ4a, TILZ4

---

Galerie de données de validation

• 

  WB analysis using 25418-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25418-1-AP (TSC22D2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 25418-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25418-1-AP (TSC22D2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25418-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25418-1-AP (TSC22D2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  IP experiment of HepG2 using 25418-1-AP

  IP result of anti-TSC22D2 (IP:25418-1-AP, 5ug; Detection:25418-1-AP 1:300) with HepG2 cells lysate 2500ug.

• 

  IHC staining of human kidney using 25418-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 25418-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 25418-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 25418-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 25418-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TSC22D2 antibody (25418-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules MDA-MB-453s, cellules SH-SY5Y
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu rénal humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

25418-1-AP cible TSC22D2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TSC22D2 Protéine recombinante Ag22294
• Nom complet: TSC22 domain family, member 2
• Masse moléculaire calculée: 780 aa, 79 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC044643
• Symbole du gène: TSC22D2
• Identification du gène (NCBI): 9819
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TSC22D2 (transforming growth factor β-stimulated clone 22 domain family, member 2) is a member of the TSC-22 domain family comprising putative transcription factors that are characterized by a carboxy-terminal leucine zipper and an adjacent TSC-box. TSC22D2 is expressed at low levels in CRC and that TSC22D2 overexpression significantly inhibited the growth rate of cancer cells (PMID: 27573352).

Publications

Species	Application	Title
human	WB,IHC	BMC Cancer; A novel cuproptosis-related gene model predicts outcomes and treatment responses in pancreatic adenocarcinoma; Authors - Qixian Liu

{{ptg:RelatedPrimaryAntibodies}}