Anticorps Polyclonal de lapin anti-GILZ

GILZ Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 12352-1-AP

Synonymes

TSC22D3, TSC22 D3, TSC 22R, DSIP-immunoreactive peptide, DSIPI

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Galerie de données de validation

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  WB analysis of mouse brain using 12352-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12352-1-AP (GILZ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12352-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12352-1-AP (GILZ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12352-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12352-1-AP (GILZ antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 12352-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12352-1-AP (GILZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 12352-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using GILZ antibody (12352-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

12352-1-AP cible GILZ dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GILZ Protéine recombinante Ag3015
• Nom complet: TSC22 domain family, member 3
• Masse moléculaire calculée: 134 aa, 15 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC018148
• Symbole du gène: GILZ
• Identification du gène (NCBI): 1831
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GILZ, also known as TSC22D3 and DSIPI, is a glucocorticoid responsive protein and is thought to mediate some of the anti-inflammatory effects of glucocorticoid receptors (GRs). GILZ is mainly localized in the cytoplasm. It is an anti-inflammatory molecule that could mediate the immunomodulatory activities of GCs and also plays a role in cell differentiation in various cells and tissues (PMID: 35011684).

Publications

Species	Application	Title
human	WB	J Adv Res; Mini and enhanced CRISPR activators for cancer therapies; Authors - Meiyu Huang
rat	WB	Invest Ophthalmol Vis Sci; Glucocorticoid-Induced Leucine Zipper Protects the Retina From Light-Induced Retinal Degeneration by Inducing Bcl-xL in Rats.; Authors - Ruiping Gu
rat	WB	Invest Ophthalmol Vis Sci; Glucocorticoid-Induced Leucine Zipper Suppresses ICAM-1 and MCP-1 Expression by Dephosphorylation of NF-κB p65 in Retinal Endothelial Cells.; Authors - Ruiping Gu
human	WB	Exp Eye Res; Glucocorticoid-induced leucine zipper overexpression inhibits lipopolysaccharide-induced retinal inflammation in rats.; Authors - Ruiping Gu

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