Anticorps Polyclonal de lapin anti-TSP50

• Phare
• Validé par KD/KO

TSP50 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF,ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 12574-1-AP

Synonymes

Cancer/testis antigen 20, CT20, PRSS50, Serine protease 50, testes protease 50, TSP50

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Galerie de données de validation

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  WB analysis of MCF-7 using 12574-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12574-1-AP (TSP50 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12574-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12574-1-AP (TSP50 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12574-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12574-1-AP (TSP50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12574-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12574-1-AP (TSP50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 12574-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12574-1-AP (TSP50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12574-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12574-1-AP (TSP50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 12574-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12574-1-AP (TSP50 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12574-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12574-1-AP (TSP50 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12574-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12574-1-AP (TSP50 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 12574-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12574-1-AP (TSP50 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 12574-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12574-1-AP (TSP50 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 12574-1-AP

  Immunofluorescent analysis of MCF-7 cells using 12574-1-AP (TSP50 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF7, cellules HEK-293, cellules MCF-7, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du sein humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below

Informations sur le produit

12574-1-AP cible TSP50 dans les applications de WB, IHC, IF,ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TSP50 Protéine recombinante Ag3247
• Nom complet: testes-specific protease 50
• Masse moléculaire calculée: 385 aa, 43 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC033016
• Symbole du gène: TSP50
• Identification du gène (NCBI): 29122
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TSP50 (Testis-specific protease-like protein 50) is also named as PRSS50, CT20 and belongs to the peptidase S1 family. It can enhance the expression of NFKB target genes involved in cell proliferation, and promotes cell proliferation, at least partially, through activation of the NFKB signalling pathway(PMID:21385156). There are two protein bands of 37 kDa and 43 kDa to be detected in human testes that the the difference is most likely caused by posttranslational proteolytic cleavage(PMID:11782390). The full length protein has a signal peptide with 39 amino acids and two glycosylation sites.

Publications

Species	Application	Title
human	WB,IHC	BMC Cancer; Testes-specific protease 50 (TSP50) promotes invasion and metastasis by inducing EMT in gastric cancer.; Authors - Qing-Hua Cao ; KD Validated
		Oncol Rep; Lentivirus-mediated knockdown of TSP50 suppresses the growth of non-small cell lung cancer cells via G0/G1 phase arrest.; Authors - Wen-Liang Qiao
human	WB,IHC	Gastroenterol Res Pract; Overexpression of Testes-Specific Protease 50 (TSP50) Predicts Poor Prognosis in Patients with Gastric Cancer.; Authors - Fang Liu
human	IHC	Oncol Lett; Testes-specific protease 50 as an independent risk factor for poor prognosis in patients with non-small cell lung cancer.; Authors - Wen-Liang Qiao