Anticorps Polyclonal de lapin anti-TTDN1

TTDN1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Avis (3)

N° de cat : 25410-1-AP

Synonymes

C7orf11, ABHS, TTD non-photosensitive 1 protein, ORF20, MPLKIP

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 25410-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25410-1-AP (TTDN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 25410-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25410-1-AP (TTDN1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3,
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

25410-1-AP cible TTDN1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TTDN1 Protéine recombinante Ag22094
• Nom complet: chromosome 7 open reading frame 11
• Masse moléculaire calculée: 179 aa, 19 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC026265
• Symbole du gène: TTDN1
• Identification du gène (NCBI): 136647
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TTDN1 colocalizes with Plk1 at the centrosome in mitosis and the midbody during cytokinesis. It is phosphorylated by Cdk1 in mitosis, and this is required for its interaction with Plk1. Overexpression of TTDN1 or its knockdown by siRNA causes multi-polar spindles and multiple nuclei, suggesting that TTDN1 plays a role in regulating mitosis and cytokinesis. Mutations in the TTDN1 gene are associated with a distinct trichothiodystrophy phenotype. (PMID:17310276, 25290684)

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (07-19-2025)

Antibodies were used for Ttdn1 detection in RKO cells! Incubation overnight at 4C

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: RKO

TTDN1 Antibody Western Blot validation (1:2000 dilution) in RKO (Cat no:25410-1-AP)

FH

Moritz (Verified Customer) (08-20-2024)

I used this antibody to detect MPLKIP/TTDN1 with Western blot in HEK293T WT cell lysate, which worked perfectly.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293T WT

TTDN1 Antibody Western Blot validation (1:3000 dilution) in HEK293T WT (Cat no:25410-1-AP)

FH

Tsimafei (Verified Customer) (08-12-2024)

Antibody detect a band in the range between 20-25 kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESCs

TTDN1 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:25410-1-AP)

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