Anticorps Polyclonal de lapin anti-TTK

• Phare
• Validé par KD/KO

TTK Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (1)

N° de cat : 10381-1-AP

Synonymes

ESK, MPS1, MPS1L1, PYT, TTK, TTK protein kinase

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Galerie de données de validation

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  WB analysis using 10381-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10381-1-AP (TTK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10381-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10381-1-AP (TTK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10381-1-AP

  WB result of TTK antibody (10381-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTK transfected HEK-293 cells.

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  IP experiment of MDA-MB-453s using 10381-1-AP

  IP Result of anti-TTK (IP:10381-1-AP, 4ug; Detection:10381-1-AP 1:300) with MDA-MB-453s cells lysate 2800ug.

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  IHC staining of human breast cancer using 10381-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10381-1-AP (TTK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 10381-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 10381-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 10381-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 10381-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 10381-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 10381-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10381-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10381-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TTK antibody (10381-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 10381-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TTK (10381-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules HEK-293, cellules HeLa, cellules MCF-7
• Résultats positifs en IP: cellules MDA-MB-453s
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du foie humain, tissu de côlon de souris, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Informations sur le produit

10381-1-AP cible TTK dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TTK Protéine recombinante Ag0405
• Nom complet: TTK protein kinase
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 96-97 kDa
• Numéro d’acquisition GenBank: BC000633
• Symbole du gène: TTK
• Identification du gène (NCBI): 7272
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mps1 family of kinases colocalizes with mitotic checkpoint proteins in kinetochores and/or spindle pole bodies/centrosomes. TTK has been shown to be a cell cycle-regulated kinase displaying maximum activity during M phase(PMID:15618221). Its kinase activity is required for proper chromosome segregation during mitosis through its involvements in microtubule-chromosome attachment error correction and the mitotic checkpoint(PMID:20937696). TTK mRNA is present at relatively high levels in testis and thymus, tissues which contain a large number of proliferating cells, but is not detected in most other benign tissues (PMID:1639825). It has 2 isoforms produced by alternative splicing (PMID:25137017).

Publications

Species	Application	Title
human	IF	J Cell Biol; PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle size; Authors - Tomoaki Sobajima
		Oxid Med Cell Longev; Oxidative Stress Delays Prometaphase/Metaphase of the First Cleavage in Mouse Zygotes via the MAD2L1-Mediated Spindle Assembly Checkpoint.; Authors - Que Wu
human	WB,IF	J Nanobiotechnology; GRP75-driven, cell-cycle-dependent macropinocytosis of Tat/pDNA-Ca2+ nanoparticles underlies distinct gene therapy effect in ovarian cancer.; Authors - Linjia Su
human	IHC	Int J Cancer; Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.; Authors - Fangqiu Fu
human	IHC	Front Oncol; Identification and Validation of Hub Genes Associated with Bladder Cancer by Integrated Bioinformatics and Experimental Assays; Authors - Kang Chen
human	IHC	Front Mol Biosci; Characteristic Analysis of Featured Genes Associated With Stemness Indices in Colorectal Cancer.; Authors - Yongqu Lu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Zhiping (Verified Customer) (07-28-2022)

Very clear band

• Applications: Western Blot
• Primary Antibody Dilution: 1:500