Anticorps Polyclonal de lapin anti-Alpha Tubulin

Alpha Tubulin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(31)

Avis (1)

N° de cat : 14555-1-AP

Synonymes

TUBA1A, Tubulin alpha 1A chain, Alpha-tubulin 3, Detyrosinated tubulin alpha-1A chain, EC:3.6.5.-

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• Galerie de données de validation
• Proteintech vs. Other Vendors

• 

  WB analysis using 14555-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14555-1-AP (Alpha Tubulin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 14555-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14555-1-AP (alpha Tubulin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 14555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14555-1-AP (Alpha Tubulin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14555-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (14555-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 14555-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (14555-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules NIH/3T3, tissu cérébral de souris, tissu de cervelet de souris, tissu rénal de rat
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 30 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

14555-1-AP cible Alpha Tubulin dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Alpha Tubulin Protéine recombinante Ag6038
• Nom complet: tubulin, alpha 1a
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC050637
• Symbole du gène: Alpha Tubulin
• Identification du gène (NCBI): 7846
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
bovine	WB	Int J Biol Macromol; NR1D1 activation alleviates inflammatory response through inhibition of IL-6 expression in bovine endometrial epithelial cells; Authors - Wanghao Yang
rat	WB	ACS Appl Mater Interfaces; Cerium-Organic Framework and Resveratrol Composite Hydrogel Scaffold with Dual Antioxidant Activity for Enhanced Bone Regeneration; Authors - Yao Lu
human	WB	mBio; Regulation of the WNT-CTNNB1 signaling pathway by severe fever with thrombocytopenia syndrome virus in a cap-snatching manner; Authors - Xia-Ming Jiang
mouse	WB	CNS Neurosci Ther; Plasma-Derived Exosomal i-tRF-LeuCAA as Biomarker for Glioma Diagnosis and Promoter of Epithelial-Mesenchymal Transition via TPM4 Regulation; Authors - Hongyu Liu
mouse	WB	J Neurosci Res; Tau nuclear translocation is a leading step in tau pathology process through P53 stabilization and nucleolar dispersion.; Authors - Shaqayeq Roqanian
human	WB	J Cell Sci; ARF6 protects sister chromatid cohesion to ensure the formation of stable kinetochore-microtubule attachments.; Authors - Mohamed Bourmoum

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yuting (Verified Customer) (08-27-2025)

Good product

• Applications: Western Blot
• Cell Tissue Type: human

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