Anticorps Polyclonal de lapin anti-Alpha Tubulin

Alpha Tubulin Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 14555-1-AP

Synonymes

alpha tubulin, TUBA1A, TUBA3


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  • Western Blot (WB) analysis of mouse brain tissue using Alpha Tubulin Polyclonal antibody (14555-1-AP)

    WB analysis of mouse brain using 14555-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14555-1-AP (alpha Tubulin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using Alpha Tubulin Polyclonal antibody (14555-1-AP)

    WB analysis of mouse cerebellum using 14555-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14555-1-AP (alpha Tubulin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Alpha Tubulin Polyclonal antibody (14555-1-AP)

    WB analysis of HeLa using 14555-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14555-1-AP (alpha Tubulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Alpha Tubulin Polyclonal antibody (14555-1-AP)

    IHC staining of mouse testis using 14555-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14555-1-AP (Alpha Tubulin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha Tubulin Polyclonal antibody (14555-1-AP)

    IF Staining of HepG2 using 14555-1-AP

    Immunofluorescent analysis of HepG2 cells using 14555-1-AP (alpha Tubulin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

"Alpha Tubulin Antibodies" Comparison

View side-by-side comparison of Alpha Tubulin antibodies from other vendors to find the one that best suits your research needs.

Applications testées

Résultats positifs en WBtissu cérébral de souris, tissu de cervelet de souris
Résultats positifs en IHCtissu testiculaire de souris,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IFcellules HepG2

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochimie (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

14555-1-AP cible Alpha Tubulin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Alpha Tubulin Protéine recombinante Ag6038
Nom complet tubulin, alpha 1a
Masse moléculaire calculée 50 kDa
Poids moléculaire observé 50-55 kDa
Numéro d’acquisition GenBankBC050637
Symbole du gène TUBA1A
Identification du gène (NCBI) 7846
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Protocole

Product Specific Protocols
WB protocol for Alpha Tubulin antibody 14555-1-APDownload protocol
IHC protocol for Alpha Tubulin antibody 14555-1-APDownload protocol
IF protocol for Alpha Tubulin antibody 14555-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Neurosci Res

Tau nuclear translocation is a leading step in tau pathology process through P53 stabilization and nucleolar dispersion.

Authors - Shaqayeq Roqanian
humanWB

J Cell Sci

ARF6 protects sister chromatid cohesion to ensure the formation of stable kinetochore-microtubule attachments.

Authors - Mohamed Bourmoum
mouseWB

Biochim Biophys Acta Mol Basis Dis

Dicer1 promotes Aβ clearance via blocking B2 RNA-mediated repression of apolipoprotein E.

Authors - Yan Wang
humanWB,IF

Exp Cell Res

Identification and characterization of INMAP, a novel interphase nucleus and mitotic apparatus protein that is involved in spindle formation and cell cycle progression.

Authors - Shen Enzhi E
humanWB

Cell Rep

SFTSV Infection Induces BAK/BAX-Dependent Mitochondrial DNA Release to Trigger NLRP3 Inflammasome Activation.

Authors - Shufen Li
humanWB

Nanomedicine

Chemosensitization and mitigation of Adriamycin-induced cardiotoxicity using combinational polymeric micelles for co-delivery of quercetin/resveratrol and resveratrol/curcumin in ovarian cancer.

Authors - Adel Al Fatease