Alpha Tubulin Polyclonal antibody

Alpha Tubulin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11224-1-AP

Synonyms

alpha Tubulin, Alpha tubulin ubiquitous, alpha tubulin,a tubulin, TUBA1B, tubulin, tubulin, alpha 1b, α-tubulin



Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, mouse colon tissue, mouse eye tissue, mouse liver tissue, mouse lung tissue, mouse uterus tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human endometrial cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, HepG2 cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:15000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11224-1-AP targets Alpha Tubulin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken, hamster, human, monkey, mouse, rat, swine, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Tubulin fusion protein Ag1727
Full Name tubulin, alpha 1b
Calculated molecular weight 50 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC009314
Gene symbol TUBA1B
Gene ID (NCBI) 10376
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

What is the function of alpha tubulin?

Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerises to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport and are also involved in cell division.

 Where is alpha-tubulin expressed?

Alpha tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton. 

What are the post-translational modifications of alpha tubulin?

The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerised mictotubule. For example, the first modification to be identified was detyrosination1, as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyronisation along the structure, regulating protein interactions and allowing subcellular compartments to be defined.2,3  Polyglutamylation also occurs on several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).

 1. Gundersen, G. G., Khawaja, S. & Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules. J. Cell Biol. 105, 251-64 (1987).

2. Galjart, N. Plus-End-Tracking Proteins and Their Interactions at Microtubule Ends. Curr. Biol. 20, R528-R537 (2010).

3. Jiang, K. & Akhmanova, A. Microtubule tip-interacting proteins: a view from both ends. Curr. Opin. Cell Biol. 23, 94-101 (2011).


Protocols

Product Specific Protocols
WB protocol for Alpha Tubulin antibody 11224-1-APDownload protocol
IHC protocol for Alpha Tubulin antibody 11224-1-APDownload protocol
IF protocol for Alpha Tubulin antibody 11224-1-APDownload protocol
IP protocol for Alpha Tubulin antibody 11224-1-APDownload protocol
FC protocol for Alpha Tubulin antibody 11224-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Br J Cancer

Interferon-alpha promotes immunosuppression through IFNAR1/STAT1 signalling in head and neck squamous cell carcinoma.

Authors - Hailong Ma
mouseWB

Int J Mol Sci

Fucoidan Alleviates Acetaminophen-Induced Hepatotoxicity via Oxidative Stress Inhibition and Nrf2 Translocation.

Authors - Yu-Qin Wang
humanWB

Dev Cell

AMPK Promotes SPOP-Mediated NANOG Degradation to Regulate Prostate Cancer Cell Stemness.

Authors - Xinbo Wang
humanWB

Onco Targets Ther

XRCC1 rs1799782 (C194T) polymorphism correlated with tumor metastasis and molecular subtypes in breast cancer.

Authors - Qing Li
mouseWB

Environ Sci Pollut Res Int

An increase of estrogen receptor α protein level regulates BDE-209-mediated blood-testis barrier disruption during spermatogenesis in F1 mice.

Authors - Jinxia Zhai
mouseWB

Proc Natl Acad Sci U S A

Cell-autonomous adiposity through increased cell surface GLUT4 due to ankyrin-B deficiency.

Authors - Damaris N Lorenzo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Chao (Verified Customer) (03-12-2020)

Good sharp clear band in western blot.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart
FH

Wei (Verified Customer) (01-30-2020)

Very good, sharp band for internal control. The protein is better than GAPDH for quantification in heart diseases.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Mouse Heart
FH

KARTHIKEYAN (Verified Customer) (11-20-2019)

I used this antibody in mouse brain endothelial cell line, it worked really well, i would definitely recommend this product.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Brain Endothelial cell line
Alpha Tubulin Antibody Western Blot, validation (1:1000 dilution) in Brain Endothelial cell line (Cat no:11224-1-AP)
FH

Pooja (Verified Customer) (11-05-2019)

This antibody works very well as loading control. Even lower dilution can be use.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: MCF7 cells
FH

Yuan (Verified Customer) (11-02-2019)

Very nice staining for A549 cells with 1:200 dilution

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: A549 cell
Alpha Tubulin Antibody Immunofluorescence, validation (1:200 dilution) in A549 cell (Cat no:11224-1-AP)
FH

Kishor (Verified Customer) (01-30-2019)

I got good results for Western Blotting (1:1000) and IF (1:300)

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1
Alpha Tubulin Antibody Western Blot,Immunofluorescence, validation (1:1000 dilution) in H69, HUCCT-1 (Cat no:11224-1-AP)
FH

kk (Verified Customer) (11-21-2018)

Excellent product, single and sharp band in all my blots

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: primary mouse islets