Anticorps Monoclonal anti-TUBB3-specific/TUJ1

TUBB3-specific/TUJ1 Monoclonal Antibody for WB, IF-P, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

Publications(2)

CloneNo.

1F8G10

Avis (1)

N° de cat : CL594-66375

Synonymes

TUBB3, TUBB3-specific, TUJ1, beta tubulin, Tubulin beta 3 chain

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Galerie de données de validation

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  WB analysis using CL594-66375

  Various lysates were subjected to SDS PAGE followed by western blot with CL594-66375 (TUBB3-specific/TUJ1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using CL594-66375

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-66375 (TUBB3-specific antibody) at dilution of 1:50.

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  IF Staining of mouse brain using CL594-66375

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-66375 (TUBB3-specific antibody) at dilution of 1:50.

• 

  IF Staining of mouse brain using CL594-66375

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 TUBB3-specific antibody (CL594-66375, Clone: 1F8G10 ) at dilution of 1:200.

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  FC experiment of SH-SY5Y using CL594-66375

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TUBB3-specific (CL594-66375, Clone:1F8G10) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules Neuro-2a, cellules PC-12
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en FC (Intra): cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

CL594-66375 cible TUBB3-specific/TUJ1 dans les applications de WB, IHC, IF-P, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tubulin, beta 3
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: NM_001197181
• Symbole du gène: TUBB3
• Identification du gène (NCBI): 10381
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms. And the antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Publications

Species	Application	Title
mouse	IF	Sci Rep; Sexual dimorphic impacts of systemic vincristine on lower urinary tract function.; Authors - Nao Iguchi
mouse	IHC	Exp Cell Res; A parasympathetic neurotransmitter induces myoepithelial cell differentiation during salivary gland development.; Authors - Yuki Shindo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Victoria (Verified Customer) (10-15-2021)

Good IF detection of differentiating neurons in tissue at a concentration of 1:100.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: E3 chick neural tube. FA fixed.

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