Anticorps Polyclonal de lapin anti-TUBGCP3

• Phare
• Validé par KD/KO

TUBGCP3 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 15719-1-AP

Synonymes

GCP 3, GCP3, h104p, hGCP3, hGrip104, hSpc98, SPBC98, Spc98p, TUBGCP3

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Galerie de données de validation

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  WB analysis of HeLa using 15719-1-AP

  WB result of TUBGCP3 antibody (15719-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-TUBGCP3 transfected HeLa cells.

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  WB analysis of Jurkat using 15719-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15719-1-AP (TUBGCP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IF: See 2 publications below

Informations sur le produit

15719-1-AP cible TUBGCP3 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TUBGCP3 Protéine recombinante Ag8437
• Nom complet: tubulin, gamma complex associated protein 3
• Masse moléculaire calculée: 103.5 kDa
• Poids moléculaire observé: 95-101 kDa
• Numéro d’acquisition GenBank: BC007763
• Symbole du gène: TUBGCP3
• Identification du gène (NCBI): 10426
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TUBGCP3, also named as γ‐tubulin complex protein 3, localized to the centrosome, is a member of γ-tubulin complex proteins which play key roles in microtubule nucleation and organization. It has been reported that TUBGCP3 rotation can bring γ-tubulin molecules into proximity. 15719-1-AP antibody detects the isoform proteins around 95-101 kDa and 50 kDa in SDS-PAGE.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; The dynamics of microtubule minus ends in the human mitotic spindle.; Authors - Nicolas Lecland
human	WB	Nat Commun; Structural mechanisms for centrosomal recruitment and organization of the microtubule nucleator γ-TuRC; Authors - Qi Gao
human	WB	Nat Commun; Modular assembly of the principal microtubule nucleator γ-TuRC.; Authors - Martin Würtz
mouse	WB	Nat Commun; Non-centrosomal nucleation mediated by augmin organizes microtubules in post-mitotic neurons and controls axonal microtubule polarity.; Authors - Carlos Sánchez-Huertas
human	WB,IF	J Cell Sci; MZT1 regulates microtubule nucleation by linking γTuRC assembly to adapter-mediated targeting and activation.; Authors - Rosa Ramírez Cota ; KD Validated
human	WB	J Cell Sci; Retromer/WASH dependent sorting of nutrient transporters requires a multivalent interaction network with ANKRD50.; Authors - Arunas Kvainickas

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