Anticorps Polyclonal de lapin anti-TWIST1-specific

• Phare
• Validé par KD/KO

TWIST1-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF/ICC, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(193)

Avis (2)

N° de cat : 25465-1-AP

Synonymes

TWIST1, ACS3, bHLHa38, BPES2, BPES3

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Galerie de données de validation

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  WB analysis using 25465-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 25465-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 25465-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 25465-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of L02 using 25465-1-AP

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using TWIST1-specific antibody (25465-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules U-251, cellules A549, cellules MDA-MB-231, cellules PC-3, cellules Raji, cellules SH-SY5Y
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du pancréas humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules L02,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 176 publications below
• IHC: See 30 publications below
• IF: See 10 publications below
• CoIP: See 2 publications below
• ChIP: See 5 publications below

Informations sur le produit

25465-1-AP cible TWIST1-specific dans les applications de WB, IHC, IF/ICC, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TWIST1-specific Protéine recombinante Ag21924
• Nom complet: twist homolog 1 (Drosophila)
• Masse moléculaire calculée: 202 aa, 21 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC036704
• Symbole du gène: TWIST1
• Identification du gène (NCBI): 7291
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Twist-related protein 1 (TWIST1), also named as BHLHA38, is 202 amino acid protein, which contains one bHLH domain. TWIST1 localizes in the nucleus and acts as a transcriptional regulator. TWIST1 inhibits monogenesis by sequestrating E proteins and inhibits tans-activation by MEF2. TWIST1 is expressed in subset of mesodermal cells. The antibody reacts specifically with Twist1 protein. The calculated molecular weight of TWIST1 is 21 kDa, but the observed molecular weight is about 30 kDa due to the post-translational modification(PMID: 30733340).

Publications

Species	Application	Title
mouse	WB	Sci Adv; Phospholipid remodeling is critical for stem cell pluripotency by facilitating mesenchymal-to-epithelial transition.; Authors - Yi Wu
mouse	WB,IHC	Theranostics; Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma; Authors - Lu-Ning Qin
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
mouse	WB,IHC	Int J Biol Macromol; Fucoidan/chitosan layered PLGA nanoparticles with melatonin loading for inducing intestinal absorption and addressing triple-negative breast cancer progression; Authors - Yu-Wei Yen
human	WB,IHC	Int J Nanomedicine; Combined miR-181a-5p and Ag Nanoparticles are Effective Against Oral Cancer in a Mouse Model; Authors - Guoqiang Xu
human	IHC	Clin Transl Med; FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression.; Authors - Lei Miao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Angie (Verified Customer) (07-26-2025)

WB showed a band below 37 kDa at dilution of 1:1000 incubated overnight at 4°C. Secondary antibody: Donkey-anti-rabbit (Alexa Fluor 680) at 1:20000 dilution incubated at room temperature for 1 hour.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: A549

TWIST1-specific Antibody Western Blot validation (1:1000 dilution) in A549 (Cat no:25465-1-AP)

FH

Pramod Bahadur (Verified Customer) (02-27-2024)

We are missing the vials so I dont have lot number.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: PC3

TWIST1-specific Antibody Western Blot validation (1:1000 dilution) in PC3 (Cat no:25465-1-AP)

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