Anticorps Polyclonal de lapin anti-ERp19

ERp19 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 20181-1-AP

Synonymes

AGR1, AIF, AIFM1, ERp18, ERp19, hAG 1, PDCD8, PDIA16, TLP19, TXNDC12

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Galerie de données de validation

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  WB analysis of human brain using 20181-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20181-1-AP (ERp19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 20181-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 20181-1-AP (ERp19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 20181-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20181-1-AP (ERp19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20181-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20181-1-AP (ERp19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 20181-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20181-1-AP (ERp19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20181-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20181-1-AP (ERp19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 20181-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20181-1-AP (ERp19 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 20181-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20181-1-AP (ERp19 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 20181-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20181-1-AP (ERp19 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 20181-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20181-1-AP (ERp19 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu cardiaque humain, tissu pulmonaire de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cirrhose hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

20181-1-AP cible ERp19 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ERp19 Protéine recombinante Ag14045
• Nom complet: thioredoxin domain containing 12 (endoplasmic reticulum)
• Masse moléculaire calculée: 172 aa, 19 kDa
• Poids moléculaire observé: 20 kDa, 40 kDa
• Numéro d’acquisition GenBank: BC001493
• Symbole du gène: TXNDC12
• Identification du gène (NCBI): 51060
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TXNDC12, which is known as thioredoxin domain-containing protein 12, ERp16, ERp18, ERp19, or hTLP19, is a member of the protein disulfide isomerase (PDI) family that plays important roles in cancer development and progression. TXNDC12 has been demonstrated to contribute to tumorigenicity in human gastric cancer by promoting cell growth, migration, and invasion(PMID: 25940440). Other studies also revealed the overexpression of TXNDC12 promotes HCC metastasis through β-catenin-mediated induction of EMT and that TXNDC12 has the potential to be a prognostic factor and therapeutic target of HCC. (PMID: 31570854)