Anticorps Polyclonal de lapin anti-Thymidylate synthase

• Phare
• Validé par KD/KO

Thymidylate synthase Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

27

N° de cat : 15047-1-AP

Synonymes

HsT422, Thymidylate synthase, thymidylate synthetase, TMS, TS, TSase, TYMS

---

Galerie de données de validation

• 

  WB analysis using 15047-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15047-1-AP

  WB result of Thymidylate synthase antibody (15047-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Thymidylate synthase transfected HEK-293 cells.

• 

  WB analysis of NIH/3T3 using 15047-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15047-1-AP (Thymidylate synthase antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 15047-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15047-1-AP

  IP Result of anti-Thymidylate synthase (IP:15047-1-AP, 3ug; Detection:15047-1-AP 1:600) with HeLa cells lysate 3200ug.

• 

  IHC staining of human endometrial cancer using 15047-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 15047-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15047-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15047-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15047-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 15047-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Thymidylate synthase (15047-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HEK-293T, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de l'endomètre humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 21 publications below
• IHC: See 5 publications below

Informations sur le produit

15047-1-AP cible Thymidylate synthase dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Thymidylate synthase Protéine recombinante Ag7027
• Nom complet: thymidylate synthetase
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 32-36 kDa
• Numéro d’acquisition GenBank: BC002567
• Symbole du gène: TYMS
• Identification du gène (NCBI): 7298
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.

Publications

Species	Application	Title
human	WB	J Pineal Res; Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.; Authors - Li Cui
		Nat Commun; CETSA screening identifies known and novel thymidylate synthase inhibitors and slow intracellular activation of 5-fluorouracil.; Authors - Helena Almqvist
human	WB	Cancer Res; 5-Fluorouracil Enhances the Antitumor Activity of the Glutaminase Inhibitor CB-839 against PIK3CA-Mutant Colorectal Cancers.; Authors - Yiqing Zhao
human	WB	Cell Rep; The mTORC1 Signaling Network Senses Changes in Cellular Purine Nucleotide Levels.; Authors - Gerta Hoxhaj
human	WB	Cell Chem Biol; CETSA interaction proteomics define specific RNA-modification pathways as key components of fluorouracil-based cancer drug cytotoxicity.; Authors - Ying Yu Liang
human	WB	Oxid Med Cell Longev; lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients.; Authors - Guibin Liang ; KD Validated

Produits associés

ERCC1 Polyclonal antibody

14586-1-AP

ERCC1 Monoclonal antibody

66275-1-Ig

MTR Polyclonal antibody

25896-1-AP

P glycoprotein Polyclonal antibody

22336-1-AP