Anticorps Polyclonal de lapin anti-Transgelin-2-specific

• Phare
• Validé par KD/KO

Transgelin-2-specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 15508-1-AP

Synonymes

TAGLN2, Transgelin 2, Transgelin-2-specific

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Galerie de données de validation

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  WB analysis of HeLa using 15508-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15508-1-AP (Transgelin-2-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15508-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15508-1-AP (Transgelin-2-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 15508-1-AP

  IP Result of anti-Transgelin-2-specific (IP:15508-1-AP, 3ug; Detection:15508-1-AP 1:500) with L02 cells lysate 1000ug.

• 

  IHC staining of human kidney using 15508-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15508-1-AP (Transgelin-2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human oesophagus cancer using 15508-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15508-1-AP (Transgelin-2-specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 15508-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15508-1-AP (Transgelin-2-specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15508-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15508-1-AP (Transgelin-2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15508-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 15508-1-AP (Transgelin-2-specific Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 15508-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 15508-1-AP (Transgelin-2-specific Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 15508-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Transgelin-2-specific antibody (15508-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 15508-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug Transgelin-2-specific antibody (15508-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293
• Résultats positifs en IP: cellules L02
• Résultats positifs en IHC: tissu de cancer de l'œsophage humain, tissu hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

15508-1-AP cible Transgelin-2-specific dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: transgelin 2
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: NM_003564
• Symbole du gène: TAGLN2
• Identification du gène (NCBI): 8407
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody can bind transgelin 2 specifically, but not trangelin 1 or 3.

Publications

Species	Application	Title
human	WB	Front Oncol; An Essential NRP1-Mediated Role for Tagln2 in Gastric Cancer Angiogenesis.; Authors - Hongwei Jin ; KD Validated
human	WB, IHC	J Gastroenterol Hepatol; The tumor-suppressive function of miR-1 by targeting LASP1 and TAGLN2 in esophageal squamous cell carcinoma.; Authors - Yan-Yan Du
human	WB	Cancer Cell Int; MiR-613 inhibits the proliferation, migration, and invasion of papillary thyroid carcinoma cells by directly targeting TAGLN2.; Authors - Yonglian Huang
human	WB,IF	PLoS One; Transgelin 2 participates in lovastatin-induced anti-angiogenic effects in endothelial cells through a phosphorylated myosin light chain-related mechanism.; Authors - Xiao Yuan Y ; KD Validated
human	WB,IP	Biochem Biophys Res Commun; Transgelin-2 contributes to proliferation and progression of hepatocellular carcinoma via regulating Annexin A2.; Authors - Jingpei Shi
human	WB	Molecules; MOBT Alleviates Pulmonary Fibrosis through an lncITPF-hnRNP-l-Complex-Mediated Signaling Pathway; Authors - Pan Xu

Produits associés

Transgelin 2 Monoclonal antibody

60044-1-Ig

transgelin/SM22 Polyclonal antibody

10493-1-AP

transgelin/SM22 Monoclonal antibody

60213-1-Ig