Anticorps Monoclonal anti-Tubulin Beta Class V

Tubulin Beta Class V Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

canin, Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

1D7B9

N° de cat : 66362-1-Ig

Synonymes

HsT1601, TUBB 5, TUBB6, Tubulin beta 6 chain, Tubulin beta class V, tubulin, beta 6

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Galerie de données de validation

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  WB analysis of HeLa using 66362-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 66362-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66362-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 66362-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 66362-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66362-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66362-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66362-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MDCK using 66362-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed MDCK cells using 66362-1-Ig(Tubulin beta class V antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellule NCCIT, cellules NIH/3T3, tissu testiculaire humain
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MDCK

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

66362-1-Ig cible Tubulin Beta Class V dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons canin, Humain, souris

• Réactivité: canin, Humain, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tubulin, beta 6
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: NM_032525
• Symbole du gène: TUBB6
• Identification du gène (NCBI): 84617
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tubulin beta class V, encoded by TUBB6, is an isotype of beta tubulin, the major component of microtubule. It is widely expressed in many tissues. In normal tissues, tubulin beta class V is focally expressed in ciliary structure, while in cancer tissues it exhibited a diffuse staining pattern. The alterations of its expression in tumors suggest tubulin beta class V may be used as a biomarker for some solid tumors.

Publications

Species	Application	Title
mouse	WB	Mediators Inflamm; LncRNA MDRL Mitigates Atherosclerosis through miR-361/SQSTM1/NLRP3 Signaling; Authors - Ling You
rat	WB	Life Sci; NPY stimulates cholesterol synthesis acutely by activating the SREBP2-HMGCR pathway through the Y1 and Y5 receptors in murine hepatocytes.; Authors - Fu Chen
mouse	WB	Dev Neurobiol; Alpha- and beta-tubulin isotypes are differentially expressed during brain development.; Authors - Torben J Hausrat
rat	IHC	BMC Genomics; Proteomics and transcriptomics profiling reveals distinct aspects of kidney stone related genes in calculi rats; Authors - Wang Zhu