Anticorps Polyclonal de lapin anti-U2AF35

• Phare
• Validé par KD/KO

U2AF35 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 10334-1-AP

Synonymes

FP793, RN, RNU2AF1, U2AF1, U2AF35, U2AFBP

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Galerie de données de validation

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  WB analysis of HeLa using 10334-1-AP

  WB result of U2AF35 antibody (10334-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-U2AF35 transfected HeLa cells.

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  WB analysis of COLO 320 using 10334-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10334-1-AP (U2AF35 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10334-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10334-1-AP (U2AF35 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10334-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10334-1-AP (U2AF35 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human prostate cancer using 10334-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10334-1-AP (U2AF35 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa
• Résultats positifs en IHC: tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below

Informations sur le produit

10334-1-AP cible U2AF35 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: U2AF35 Protéine recombinante Ag0399
• Nom complet: U2 small nuclear RNA auxiliary factor 1
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC001923
• Symbole du gène: U2AF1
• Identification du gène (NCBI): 7307
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

U2 auxiliary factor (U2AF), comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2 (RNU2) small nuclear RNA auxiliary factor 1 (U2AF1,synonyms: RN, FP793, U2AF35, U2AFBP, RNU2AF1)is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. The U2AF1 gene is localized to chromosome 21q22.3, which is the critical region for three diseases, progressive myoclonus epilepsy, autoimmune polyglandular disease type 1, and one form of bipolar affective disorder.

Publications

Species	Application	Title
human	WB	Mol Cell; U2AF35(S34F) Promotes Transformation by Directing Aberrant ATG7 Pre-mRNA 3' End Formation.; Authors - Sung Mi Park
human	WB	Nucleic Acids Res; PUF60-activated exons uncover altered 3' splice-site selection by germline missense mutations in a single RRM.; Authors - Jana Královicová
human	WB	Cancer Res; PHF5A Epigenetically Inhibits Apoptosis to Promote Breast Cancer Progression.; Authors - Yi-Zi Zheng
human		Nucleic Acids Res; Identification of U2AF(35)-dependent exons by RNA-Seq reveals a link between 3' splice-site organization and activity of U2AF-related proteins.; Authors - Jana Kralovicova
human	WB	Int J Mol Sci; Splicing Enhancers at Intron-Exon Borders Participate in Acceptor Splice Sites Recognition.; Authors - Tatiana Kováčová
human	WB	J Biol Chem; Splicing factor SPF30 bridges an interaction between the prespliceosome protein U2AF35 and tri-small nuclear ribonucleoprotein protein hPrp3.; Authors - Little John T JT