Anticorps Polyclonal de lapin anti-UBE2B

• Phare
• Validé par KD/KO

UBE2B Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 10733-1-AP

Synonymes

E2 17kDa, HHR6B, HR6B, RAD6 homolog B, RAD6B, UBC2, UBE2B, Ubiquitin carrier protein B, Ubiquitin protein ligase B

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Galerie de données de validation

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  WB analysis of L02 using 10733-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10733-1-AP (UBE2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 10733-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 10733-1-AP (UBE2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 10733-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10733-1-AP (UBE2B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10733-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10733-1-AP (UBE2B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules HEK-293 transfectées
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

10733-1-AP cible UBE2B dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBE2B Protéine recombinante Ag1147
• Nom complet: ubiquitin-conjugating enzyme E2B (RAD6 homolog)
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC005979
• Symbole du gène: UBE2B
• Identification du gène (NCBI): 7320
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UBE2B (Ubiquitin-conjugating enzyme E2 B) is also named as RAD6B and belongs to the ubiquitin-conjugating enzyme family. Rad6B is an E2-conjugating enzyme, reported to mediate beta-catenin polyubiquitination and stabilization, thus activating the Wnt signaling pathway (PMID:21118991). The gene encodes a 152 amino acid protein. The Rad6B and Cdc34 proteins are significantly elevated in meiotic and postmeiotic haploid germ cells when chromatin modifications occur (PMID:10373550).

Publications

Species	Application	Title
mouse	WB,IF	Biol Reprod; Identification of Ube2b as a Novel Target of Androgen Receptor in Mouse Sertoli Cells.; Authors - Mou Lisha L ; KD Validated
		Int J Mol Med; Identification of NR0B1 as a novel androgen receptor co-repressor in mouse Sertoli cells.; Authors - Yu-Chi Li
human	WB,IHC,IF	Bioengineered; UBE2B promotes ovarian cancer growth via promoting RAD18 mediated ZMYM2 monoubiquitination and stabilization.; Authors - Xi Zeng ; KD Validated
human	WB	Nat Commun; Auranofin targets UBA1 and enhances UBA1 activity by facilitating ubiquitin trans-thioesterification to E2 ubiquitin-conjugating enzymes; Authors - Wenjing Yan

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