Anticorps Polyclonal de lapin anti-UBE2L3

• Phare
• Validé par KD/KO

UBE2L3 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 14415-1-AP

Synonymes

E2 F1, L UBC, UBCE7, UBCH7, UbcM4, UBE2L3, Ubiquitin carrier protein L3, Ubiquitin protein ligase L3

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Galerie de données de validation

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  WB analysis of HeLa using 14415-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 14415-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14415-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14415-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14415-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 14415-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14415-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 14415-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14415-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 14415-1-AP

  IP Result of anti-UBE2L3 (IP:14415-1-AP, 3ug; Detection:14415-1-AP 1:2000) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human lung cancer using 14415-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14415-1-AP (UBE2L3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14415-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14415-1-AP (UBE2L3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HEK-293, cellules K-562, cellules MCF-7, cellules NIH/3T3, tissu rénal de rat, tissu rénal de souris, tissu testiculaire de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IP: See 3 publications below

Informations sur le produit

14415-1-AP cible UBE2L3 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBE2L3 Protéine recombinante Ag5758
• Nom complet: ubiquitin-conjugating enzyme E2L 3
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC053368
• Symbole du gène: UBE2L3
• Identification du gène (NCBI): 7332
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UBE2L3(Ubiquitin-conjugating enzyme E2 L3) is also named as UBCE7, UBCH7 and belongs to the ubiquitin-conjugating enzyme family. It regulates nuclear hormone receptors transcriptional activity and may play a role in myelopoiesis.UBE2L3 is associated with susceptibility to systemic lupus erythematosus (SLE) and rheumatoid arthritis in European ancestry populations(PMID:22045845).

Publications

Species	Application	Title
human	WB,IP	Bioengineered; E2F transcription factor 1 (E2F1) promotes the transforming growth factor TGF-β1 induced human cardiac fibroblasts differentiation through promoting the transcription of CCNE2 gene; Authors - Rongheng Liao ; KD Validated
human	WB	J Cell Sci; A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently.; Authors - Fabienne C Fiesel
pig	WB	Front Immunol; Foot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule Assembly via Post-Translational Mechanisms.; Authors - Xu Ye
pig	WB	Mol Cell Proteomics; The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators; Authors - Nitchakarn Kaokhum
mouse	WB,IHC	Cell Commun Signal; Metformin alleviates bone loss in ovariectomized mice through inhibition of autophagy of osteoclast precursors mediated by E2F1; Authors - Xudong Xie
human	WB	Mediators Inflamm; UBC Mediated by SEPT6 Inhibited the Progression of Prostate Cancer.; Authors - Ruochen Zhang ; KD Validated