Anticorps Polyclonal de lapin anti-UBC13

UBC13 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(6)

Avis (1)

N° de cat : 10243-1-AP

Synonymes

UBE2N, UbcH13, UbcH ben, EC:2.3.2.23, E2 ubiquitin-conjugating enzyme N

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Galerie de données de validation

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  WB analysis of mouse thymus using 10243-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10243-1-AP (UBC13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10243-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10243-1-AP (UBC13 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 10243-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10243-1-AP (UBC13 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10243-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10243-1-AP (UBC13 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 10243-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10243-1-AP (UBC13 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10243-1-AP

  IP result of anti-UBC13 (IP:10243-1-AP, 4ug; Detection:10243-1-AP 1:600) with MCF-7 cells lysate 1280ug.

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  IHC staining of human colon using 10243-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10243-1-AP (UBC13 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10243-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10243-1-AP (UBC13 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 10243-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10243-1-AP (UBC13 antibody at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu de thymus de souris, cellules Jurkat, tissu cardiaque humain, tissu d'intestin grêle de souris
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

10243-1-AP cible UBC13 dans les applications de WB, IHC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBC13 Protéine recombinante Ag0292
• Nom complet: ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC000396
• Symbole du gène: UBC13/UBE2N
• Identification du gène (NCBI): 7334
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2N is a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.

Publications

Species	Application	Title
monkey	WB	Alzheimers Dement; Suppressing UBE2N ameliorates Alzheimer's disease pathology through the clearance of amyloid beta; Authors - Chen Zhang
mouse	WB	Cell Mol Immunol; The deubiquitinase OTUB1 augments NF-κB-dependent immune responses in dendritic cells in infection and inflammation by stabilizing UBC13.; Authors - Floriana Mulas
pig	WB	Mol Cell Proteomics; The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators; Authors - Nitchakarn Kaokhum
mouse	WB,IP	Nat Commun; FTO-dependent m6A modification of Plpp3 in circSCMH1-regulated vascular repair and functional recovery following stroke; Authors - Bin Li
human	WB,IHC	Mol Biomed; SP1 transcriptionally regulates UBE2N expression to promote lung adenocarcinoma progression; Authors - Jianjun Li
human	WB,ChIP	Proc Natl Acad Sci U S A; Nonproteolytic ubiquitination regulates chromatin occupancy by the NCoR/SMRT/HDAC3 corepressor complex in MCF-7 breast cancer cells; Authors - Giulio Ferrero

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jessica (Verified Customer) (11-14-2019)

Antibody works well for detecting the endogenous Ubc13 protein in 293T cells. Strong signal and few non specific bands.

• Applications: Western Blot,
• Cell Tissue Type: 293T cells

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