Anticorps Polyclonal de lapin anti-UBE2V1

UBE2V1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 10207-2-AP

Synonymes

CIR1, CROC 1, CROC1, UBE2V, UBE2V1, UEV 1, UEV1, UEV1A

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Galerie de données de validation

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  WB analysis of human spleen using 10207-2-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 10207-2-AP (UBE2V1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 10207-2-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10207-2-AP (UBE2V1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu splénique humain, cellules BxPC-3

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

10207-2-AP cible UBE2V1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBE2V1 Protéine recombinante Ag0278
• Nom complet: ubiquitin-conjugating enzyme E2 variant 1
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC000468
• Symbole du gène: UBE2V1
• Identification du gène (NCBI): 7335
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UBE2V1(Ubiquitin-conjugating enzyme E2 variant 1) is also named as CROC1, UBE2V, UEV1 and belongs to the ubiquitin-conjugating enzyme family. It has no ubiquitin ligase activity on its own and the UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. CROC1 transcripts in all human tissues examined, with highest levels in brain, skeletal muscle, and kidney(PMID:9305758). It has 5 isoforms produced by alternative splicing.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sarah (Verified Customer) (09-05-2025)

Antibody signal was quite weak in HEK293T cells, including some off-target products as well. Antibody was used in 5% BSA in milk with overnight incubation at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: HEK293T

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