Anticorps Polyclonal de lapin anti-UBE4B

• Phare
• Validé par KD/KO

UBE4B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 18148-1-AP

Synonymes

EC:2.3.2.27, HDNB1, KIAA0684, UBOX3, UFD2

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Galerie de données de validation

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  WB analysis using 18148-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18148-1-AP (UBE4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18148-1-AP

  WB result of UBE4B antibody (18148-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBE4B transfected HeLa cells.

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  WB analysis of mouse testis using 18148-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18148-1-AP (UBE4B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18148-1-AP

  IP result of anti-UBE4B (IP:18148-1-AP, 4ug; Detection:18148-1-AP 1:1000) with HeLa cells lysate 2320 ug.

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  IHC staining of human stomach cancer using 18148-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18148-1-AP (UBE4B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 18148-1-AP

  Immunofluorescent analysis of HeLa cells using 18148-1-AP (UBE4B antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules K-562, cellules Raji, tissu testiculaire de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

18148-1-AP cible UBE4B dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBE4B Protéine recombinante Ag12836
• Nom complet: ubiquitination factor E4B (UFD2 homolog, yeast)
• Masse moléculaire calculée: 1173 aa, 133 kDa
• Poids moléculaire observé: 146 kDa
• Numéro d’acquisition GenBank: BC093696
• Symbole du gène: UBE4B
• Identification du gène (NCBI): 10277
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UBE4B (Ubiquitin Conjugation E4 B) is a protein-coding gene that plays a crucial role in the ubiquitination process, which is essential for protein degradation and regulation of various cellular processes. UBE4B functions as both an E3 and E4 ubiquitin ligase. It collaborates with other ubiquitin ligases to catalyze the transfer of ubiquitin molecules to target proteins, leading to their degradation or other intracellular sorting events. For example, UBE4B has been shown to ubiquitinate and degrade proteins such as Ku70, c-FLIPL, and p53. It also interacts with the E3 ubiquitin ligase ITCH to induce polyubiquitination and proteasomal degradation of target proteins.

Publications

Species	Application	Title
human	WB	Mol Cell; RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins.; Authors - Xianyan Hu
human	WB	Mol Carcinog; Expression and prognostic role of ubiquitination factor E4B in primary hepatocellular carcinoma.; Authors - Xiao-Fei Zhang
human	WB	Proteomics; SILAC-based Quantitative Proteomic Analysis of Secretome between Activated and Reverted Hepatic Stellate Cells.; Authors - Hongyu Zhang
human	WB,IHC	Onco Targets Ther; The Roles of Ubiquitination Factor E4B (UBE4B) in the Postoperative Prognosis of Patients with Renal Cell Carcinoma and in Renal Tumor Cells Growth and Metastasis.; Authors - Xu-Qiong Huang ; KD Validated
human	IHC	Pathol Res Pract; UBE4B promotes the development of lung adenocarcinoma by enhancing proliferation, migration and glycolysis via PP2A/AKT signaling.; Authors - Song Wu
human	WB	Biochim Biophys Acta Gene Regul Mech; UBE4A catalyzes NRF1 ubiquitination and facilitates DDI2-mediated NRF1 cleavage; Authors - Xianyan Hu ; KD Validated

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