Anticorps Polyclonal de lapin anti-UBL4A

• Phare
• Validé par KD/KO

UBL4A Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (3)

N° de cat : 14253-1-AP

Synonymes

DX254E, DXS254E, G6PD, GDX, ubiquitin like 4A, Ubiquitin like protein 4A, Ubiquitin like protein GDX, UBL4, UBL4A

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Galerie de données de validation

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  WB analysis of HeLa using 14253-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14253-1-AP (UBL4A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 14253-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 14253-1-AP (UBL4A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 14253-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14253-1-AP (UBL4A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14253-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14253-1-AP (UBL4A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14253-1-AP

  IP Result of anti-UBL4A (IP:14253-1-AP, 4ug; Detection:14253-1-AP 1:500) with mouse testis tissue lysate 4400ug.

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  IHC staining of human ovary tumor using 14253-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14253-1-AP (UBL4A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary tumor using 14253-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14253-1-AP (UBL4A antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 14253-1-AP

  Immunofluorescent analysis of HepG2 cells, using UBL4A antibody 14253-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HUVEC, tissu cérébral humain, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Informations sur le produit

14253-1-AP cible UBL4A dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBL4A Protéine recombinante Ag5526
• Nom complet: ubiquitin-like 4A
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC043346
• Symbole du gène: UBL4A
• Identification du gène (NCBI): 8266
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IF	J Immunol; UBL4A Augments Innate Immunity by Promoting the K63-Linked Ubiquitination of TRAF6.; Authors - Shu-Jie Peng ; KD Validated
human	WB	J Virol; SGTA-dependent regulation of Hsc70 promotes cytosol entry of SV40 from the ER.; Authors - Allison Dupzyk
pig	WB	J Nutr; Intermittent Bolus Compared With Continuous Feeding Enhances Insulin and Amino Acid Signaling to Translation Initiation in Skeletal Muscle of Neonatal Pigs.; Authors - Agus Suryawan
human	WB	J Inherit Metab Dis; Mutations in GET4 disrupt the transmembrane domain recognition complex pathway.; Authors - Mitali A Tambe
pig	WB	Pediatr Res; Preterm birth alters the feeding-induced activation of Akt signaling in the muscle of neonatal piglets; Authors - Agus Suryawan
human	WB,IF,IP	J Exp Clin Cancer Res; UBL4A inhibits autophagy-mediated proliferation and metastasis of pancreatic ductal adenocarcinoma via targeting LAMP1.; Authors - Hongze Chen ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

WEI (Verified Customer) (03-08-2022)

Weak specific band around the target size

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart

FH

WEI (Verified Customer) (10-21-2020)

This antibody recognizes a sharp, clear band around 16kDa from mouse hearts.

• Applications: Western Blot
• Cell Tissue Type: Mouse Heart

FH

Tom (Verified Customer) (10-09-2020)

This antibody specifically recognizes mouse UBL4A protein. The band was approximately 17kD.

• Applications: Western Blot