Anticorps Polyclonal de lapin anti-UGCG

• Phare
• Validé par KD/KO

UGCG Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, IP, ELISA

Conjugaison

Non conjugué

Publications(13)

Avis (2)

N° de cat : 12869-1-AP

Synonymes

Glycosylceramide synthase, Glucosylceramide synthase, GLCT-1, GCS, EC:2.4.1.80

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Galerie de données de validation

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  WB analysis of A375 using 12869-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12869-1-AP (UGCG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 12869-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 12869-1-AP (UGCG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 12869-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 12869-1-AP (UGCG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12869-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12869-1-AP (UGCG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A375 using 12869-1-AP

  IP result of anti-UGCG (IP:12869-1-AP, 4ug; Detection:12869-1-AP 1:500) with A375 cells lysate 3200ug.

• 

  IHC staining of human lung cancer using 12869-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12869-1-AP (UGCG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12869-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12869-1-AP (UGCG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12869-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12869-1-AP (UGCG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12869-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12869-1-AP (UGCG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12869-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 12869-1-AP (UGCG antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of mouse kidney using 12869-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using UGCG antibody (12869-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 12869-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using UGCG antibody (12869-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 12869-1-AP

  Immunofluorescent analysis of frozen mouse kidney tissue fixed with 4% PFA for 24 hours, embedded in OCT, permeabilized with 0.5% Triton X-100 PBS and subjected to antigen retrieval in sodium citrate buffer (pH 6.0) using UGCG antibody (12869-1-AP) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules C6, cellules U-251, tissu cérébral de souris
• Résultats positifs en IP: cellules A375
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du sein humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal de souris, tissu cérébral de souris
• Résultats positifs en IF-Fro: tissu rénal de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 5 publications below

Informations sur le produit

12869-1-AP cible UGCG dans les applications de WB, IHC, IF-P, IF-Fro, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UGCG Protéine recombinante Ag3530
• Nom complet: UDP-glucose ceramide glucosyltransferase
• Masse moléculaire calculée: 394 aa, 45 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC038711
• Symbole du gène: UGCG
• Identification du gène (NCBI): 7357
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UGCG, also named as GCS and GLCT-1, belongs to the glycosyltransferase 2 family. It catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. UGCG may also serve as a "flippase". It is up-regulated at the transcriptional level during keratinocyte differentiation. UGCG is a membrane protein. The calculated MW is 45 kDa. In SDS-PAGE, it is about 50-55 kDa.

Publications

Species	Application	Title
human	WB	Nat Commun; Glycolysis regulates KRAS plasma membrane localization and function through defined glycosphingolipids; Authors - Junchen Liu
human,mouse	WB	Cancer Res; Aneuploid cell survival relies upon sphingolipid homeostasis.; Authors - Yun-Chi Tang ; KD Validated
mouse	WB,IF	Cell Mol Biol Lett; UGCG modulates heart hypertrophy through B4GalT5-mediated mitochondrial oxidative stress and the ERK signaling pathway; Authors - Shengyu Cui
human, mouse	WB,IHC	Hum Mol Genet; Amyotrophic lateral sclerosis and denervation alter sphingolipids and up-regulate glucosylceramide synthase.; Authors - Alexandre Henriques
human	WB,IHC	Aging (Albany NY); Identification of long-term survival-associated gene in breast cancer.; Authors - Shipeng Ning
human	IF	iScience; Liver sphingomyelin synthase 1 deficiency causes steatosis, steatohepatitis, fibrosis, and tumorigenesis: An effect of glucosylceramide accumulation.; Authors - Zhiqiang Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Komuraiah (Verified Customer) (07-29-2025)

Ugcg (Rb) polyclonal antibody works great for western blot. WE will include in our forthcoming publication along with citation from this proteintech antibody. now we are also trying it for immunofluorescence.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation, Cell culture
• Primary Antibody Dilution: 1/1000

FH

Will (Verified Customer) (01-31-2019)

good antibody, would order again

• Applications: Western Blot,

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