Anticorps Polyclonal de lapin anti-UHRF1

• Phare
• Validé par KD/KO

UHRF1 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

19

N° de cat : 21402-1-AP

Synonymes

hNP95, HuNp95, ICBP90, Nuclear protein 95, RING finger protein 106, RNF106, Transcription factor ICBP90, UHRF1

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Galerie de données de validation

• 

  WB analysis of HeLa using 21402-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21402-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21402-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21402-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21402-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21402-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21402-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21402-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21402-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21402-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21402-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21402-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21402-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 21402-1-AP

  IP Result of anti-UHRF1 (IP:21402-1-AP, 4ug; Detection:21402-1-AP 1:1000) with HeLa cells lysate 3200ug.

• 

  IF Staining of HepG2 using 21402-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UHRF1 antibody (21402-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 3 publications below
• RIP: See 1 publications below

Informations sur le produit

21402-1-AP cible UHRF1 dans les applications de WB, RIP, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UHRF1 Protéine recombinante Ag16111
• Nom complet: ubiquitin-like with PHD and ring finger domains 1
• Masse moléculaire calculée: 806 aa, 91 kDa
• Poids moléculaire observé: 91 kDa
• Numéro d’acquisition GenBank: BC113875
• Symbole du gène: UHRF1
• Identification du gène (NCBI): 29128
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin-protein ligase. It may participate in methylation-dependent transcriptional regulation. UHRF1 binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. It is important for G1/S transition and may be involved in DNA repair and chromosomal stability. UHRF1's ability to repress its direct target gene expression is dependent on PHD(UHRF1) binding to unmodified H3R2. In addition, UHRF1 is a novel metabolic guardian restricting AMPK activity(PMID: 34907338).

Publications

Species	Application	Title
human	WB,IF	Nucleic Acids Res; SET8 prevents excessive DNA methylation by methylation-mediated degradation of UHRF1 and DNMT1.; Authors - Huifang Zhang
human	WB	Theranostics; A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis.; Authors - Qi Zhang
human	WB	Cell Discov; USP7 negatively controls global DNA methylation by attenuating ubiquitinated histone-dependent DNMT1 recruitment.; Authors - Jialun Li ; KD Validated
human	WB	Int J Mol Sci; Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.; Authors - Federica Morani
mouse	CoIP,RIP	Stem Cell Reports; UHRF1 interacts with snRNAs and regulates alternative splicing in mouse spermatogonial stem cells.; Authors - Shumin Zhou
	WB,IHC	Inflammation; Upregulation of UHRF1 Promotes PINK1-mediated Mitophagy to Alleviates Ferroptosis in Diabetic Nephropathy; Authors - Hongfei Ji