Anticorps Polyclonal de lapin anti-URM1

• Phare
• Validé par KD/KO

URM1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 15285-1-AP

Synonymes

C9orf74, URM1

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Galerie de données de validation

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  WB analysis using 15285-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 15285-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15285-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15285-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 15285-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15285-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 15285-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15285-1-AP

  IP result of anti-URM1 (IP:15285-1-AP, 4ug; Detection:15285-1-AP 1:500) with HepG2 cells lysate 3360 ug.

• 

  IHC staining of mouse liver using 15285-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15285-1-AP (URM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15285-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15285-1-AP (URM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 15285-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15285-1-AP (URM1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 15285-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15285-1-AP (URM1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 15285-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15285-1-AP (URM1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 15285-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15285-1-AP (URM1 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HepG2, tissu hépatique de souris, tissu placentaire humain
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu hépatique de souris, tissu de cirrhose hépatique humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

15285-1-AP cible URM1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: URM1 Protéine recombinante Ag7448
• Nom complet: ubiquitin related modifier 1 homolog (S. cerevisiae)
• Masse moléculaire calculée: 11 kDa
• Poids moléculaire observé: 11 kDa
• Numéro d’acquisition GenBank: BC003581
• Symbole du gène: URM1
• Identification du gène (NCBI): 81605
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Mol Cell Biol; Human AlkB homolog ABH8 Is a tRNA methyltransferase required for wobble uridine modification and DNA damage survival.; Authors - Fu Dragony D ; KD Validated
human	WB	Hum Mol Genet; Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients.; Authors - Ki Soon Kim

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ana Paula (Verified Customer) (08-03-2022)

mESC derived motor neurons 1:1000 primary ab in blocking buffer ON at 4 C 1:10000 secondary ab in TBS 1h at RT

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESC derived motor neurons

URM1 Antibody Western Blot validation (1:1000 dilution) in mESC derived motor neurons (Cat no:15285-1-AP)