Anticorps Polyclonal de lapin anti-USP15

• Phare
• Validé par KD/KO

USP15 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(22)

Avis (1)

N° de cat : 14354-1-AP

Synonymes

Deubiquitinating enzyme 15, EC:3.4.19.12, KIAA0529, Ubiquitin carboxyl-terminal hydrolase 15, Ubiquitin thioesterase 15

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Galerie de données de validation

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  WB analysis of HeLa using 14354-1-AP

  WB result of USP15 antibody (14354-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP15 transfected HeLa cells.

• 

  WB analysis of mouse brain using 14354-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14354-1-AP (USP15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14354-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14354-1-AP (USP15 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14354-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14354-1-AP (USP15 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14354-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14354-1-AP (USP15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14354-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14354-1-AP (USP15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14354-1-AP

  IP result of anti-USP15 (IP:14354-1-AP, 4ug; Detection:14354-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human breast cancer using 14354-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14354-1-AP (USP15 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 14354-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14354-1-AP (USP15 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human placenta using 14354-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14354-1-AP (USP15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 14354-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14354-1-AP (USP15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14354-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14354-1-AP (USP15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 14354-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 14354-1-AP (USP15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 14354-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14354-1-AP (USP15 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human ovary tumor using 14354-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14354-1-AP (USP15 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules K-562, cellules MCF-7, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu placentaire humain, tissu de cancer du sein humain, tissu de tumeur ovarienne humain, tissu d'intestin grêle de rat, tissu pulmonaire humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 12 publications below
• WB: See 19 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Informations sur le produit

14354-1-AP cible USP15 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP15 Protéine recombinante Ag5703
• Nom complet: ubiquitin specific peptidase 15
• Masse moléculaire calculée: 112 kDa
• Poids moléculaire observé: 112 kDa
• Numéro d’acquisition GenBank: BC020688
• Symbole du gène: USP15
• Identification du gène (NCBI): 9958
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin is a highly conserved eukaryotic protein that is synthesized as a fusion protein precursor, either to itself or to one of two ribosomal proteins. USP15, is also named as KIAA0529,Unph-2, Unph4, encoding a human ubiquitin-specific protease (USP). The USP15 protein contains the highly conserved Cys and His boxes present in all members of he UBP family of deubiquitinating enzymes(PMID:10444327).It has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Nat Immunol; USP15 stabilizes MDM2 to mediate cancer-cell survival and inhibit antitumor T cell responses.; Authors - Qiang Zou ; KO Validated
mouse,human	WB	Nat Commun; ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy; Authors - Xiangling Xiao ; KD Validated
mouse	WB	Mol Cell; RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages; Authors - Teodorus Theo Susanto
human	WB	Autophagy; USP33 deubiquitinates PRKN/parkin and antagonizes its role in mitophagy.; Authors - Kaifeng Niu ; KD Validated
mouse	WB	Autophagy; LncRNA 2810403D21Rik/Mirf promotes ischemic myocardial injury by regulating autophagy through targeting Mir26a.; Authors - Haihai Liang
human	WB	Leukemia; The deubiquitinase USP15 modulates cellular redox and is a therapeutic target in acute myeloid leukemia; Authors - Madeline Niederkorn

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Amy (Verified Customer) (11-08-2023)

Clean band for WB, used at a dilution lower than in the data sheet for U2OS cell lysates (1:500).

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: U2OS

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