Anticorps Recombinant de lapin anti-USP8

USP8 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240016D3

N° de cat : 83172-2-RR

Synonymes

240016D3, EC:3.4.19.12, hUBPy, HumORF8, KIAA0055

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Galerie de données de validation

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  WB analysis using 83172-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83172-2-RR (USP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 83172-2-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of U2OS using 83172-2-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 83172-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of A549 using 83172-2-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug USP8 Recombinant antibody (83172-2-RR, Clone:240016D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83172-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83172-2-RR against Human USP8 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules K-562, cellules MCF-7
• Résultats positifs en IF/ICC: cellules U2OS, cellules HeLa
• Résultats positifs en FC (Intra): cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83172-2-RR cible USP8 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: USP8 Protéine recombinante Ag27143
• Nom complet: ubiquitin specific peptidase 8
• Masse moléculaire calculée: 1118 aa, 128 kDa
• Poids moléculaire observé: 128 kDa
• Numéro d’acquisition GenBank: BC110590
• Symbole du gène: USP8
• Identification du gène (NCBI): 9101
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

USP8 is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage.

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