Anticorps Polyclonal de lapin anti-VANGL2

• Phare
• Validé par KD/KO

VANGL2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(15)

Avis (3)

N° de cat : 21492-1-AP

Synonymes

STB1, LTAP, LPP1, Loop-tail protein 1 homolog, Loop tail protein 1 homolog

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Galerie de données de validation

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  WB analysis using 21492-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21492-1-AP (VANGL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 21492-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21492-1-AP (VANGL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 21492-1-AP

  IP result of anti-VANGL2 (IP:21492-1-AP, 4ug; Detection:21492-1-AP 1:500) with HepG2 cells lysate 1880 ug.

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  IHC staining of human stomach using 21492-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21492-1-AP (VANGL2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 21492-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21492-1-AP (VANGL2 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 21492-1-AP

  Immunofluorescent analysis of HepG2 cells using 21492-1-AP (VANGL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

21492-1-AP cible VANGL2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VANGL2 Protéine recombinante Ag15833
• Nom complet: vang-like 2 (van gogh, Drosophila)
• Masse moléculaire calculée: 521 aa, 60 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC103920
• Symbole du gène: VANGL2
• Identification du gène (NCBI): 57216
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vangl2 is a key component of the planar cell polarity (PCP) pathway. Vangl2 is tightly associated with the postsynaptic density (PSD) fraction and forms a protein complex with PSD-95 and NMDA receptors. Vangl2 directly binds to the third PDZ domain of PSD-95 via its C-terminal TSV motif. Vangl2 directly binds to N-cadherin.

Publications

Species	Application	Title
mouse	WB	Adv Sci (Weinh); Isolation and Comprehensive Analysis of Cochlear Tissue-Derived Small Extracellular Vesicles; Authors - Pei Jiang
mouse	IF,WB	Dev Cell; Vangl2 limits chaperone-mediated autophagy to balance osteogenic differentiation in mesenchymal stem cells.; Authors - Yan Gong ; KD Validated
human	WB,IF	Cancer Lett; A complex of Wnt/planar cell polarity signaling components Vangl1 and Fzd7 drives glioblastoma multiforme malignant properties; Authors - Courtney A Dreyer
	IF	Sci Signal; The Helicobacter pylori CagA oncoprotein disrupts Wnt/PCP signaling and promotes hyperproliferation of pyloric gland base cells; Authors - Atsushi Takahashi-Kanemitsu
human	CoIP	Exp Cell Res; VANGL2 interacts with integrin αv to regulate matrix metalloproteinase activity and cell adhesion to the extracellular matrix.; Authors - Tammy N Jessen
	WB	FASEB J; Folate regulation of planar cell polarity pathway and F-actin through folate receptor alpha; Authors - Xiao Han

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nin (Verified Customer) (11-07-2024)

A good antibody to detect endogenous VANGL although there are nonspecific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse brain lysate

VANGL2 Antibody Western Blot validation (1:1000 dilution) in mouse brain lysate (Cat no:21492-1-AP)

FH

Stephen (Verified Customer) (05-31-2023)

works in chicken tissue fixed with 4% PFA for 2 hours, perm 0.3% tx100 for 2h primary incubated 1:200 for 24h @4 degrees,

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: chicken - embryonic auditory epithelia

VANGL2 Antibody Immunofluorescence validation (1:200 dilution) in chicken - embryonic auditory epithelia (Cat no:21492-1-AP)

FH

Louisiane (Verified Customer) (08-11-2022)

Tissues (small intestine sections) were incubated with primary antibody over the weekend at 4C and with secondary antibody overnight at 4C. The signal looks good but there is a bit of background

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 to 100

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