Anticorps Polyclonal de lapin anti-VCAM-1

• Phare
• Validé par KD/KO

VCAM-1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

83

N° de cat : 11444-1-AP

Synonymes

CD106, INCAM 100, V CAM 1, VCAM 1, VCAM1, VCAM-1

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Galerie de données de validation

• 

  WB analysis using 11444-1-AP

  Untreated or TNF-a-treated HUVEC cells were subjected to SDS PAGE followed by western blot with 11444-1-AP (VCAM-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11444-1-AP (VCAM-1 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11444-1-AP (VCAM-1 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11444-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: TNF alpha treated HUVEC cells,
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer du poumon humain, tissu placentaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• IHC: See 21 publications below
• IF: See 19 publications below
• FC: See 1 publications below

Informations sur le produit

11444-1-AP cible VCAM-1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VCAM-1 Protéine recombinante Ag1993
• Nom complet: vascular cell adhesion molecule 1
• Masse moléculaire calculée: 739 aa, 81 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC017276
• Symbole du gène: VCAM1
• Identification du gène (NCBI): 7412
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

Publications

Species	Application	Title
	IF	Cardiovasc Res; HDAC4 regulates vascular inflammation via activation of autophagy.; Authors - Di Yang
human	IF	EBioMedicine; Interactions between neutrophil extracellular traps and activated platelets enhance procoagulant activity in acute stroke patients with ICA occlusion.; Authors - Peng Zhou
	IHC	Aging Dis; Sterol-resistant SCAP Overexpression in Vascular Smooth Muscle Cells Accelerates Atherosclerosis by Increasing Local Vascular Inflammation through Activation of the NLRP3 Inflammasome in Mice.; Authors - Danyang Li
human		Br J Pharmacol; Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.; Authors - Chuanrui Ma
	IF	Br J Pharmacol; Substitution of the SERCA2 Cys(674) reactive thiol accelerates atherosclerosis by inducing endoplasmic reticulum stress and inflammation; Authors - Hang Su
		Theranostics; Ligand Activation of PPARγ by Ligustrazine Suppresses Pericyte Functions of Hepatic Stellate Cells via SMRT-Mediated Transrepression of HIF-1α.; Authors - Feng Zhang

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