Anticorps Polyclonal de lapin anti-VCP

• Phare
• Validé par KD/KO

VCP Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

36

N° de cat : 10736-1-AP

Synonymes

15S Mg(2+) ATPase p97 subunit, IBMPFD, p97, TER ATPase, TERA, valosin containing protein, VCP

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Galerie de données de validation

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  WB analysis of U2OS using 10736-1-AP

  U2OS (WT and VCP KO) lysates prepared with RIPA buffer, 10μg protein loaded. 10736-1-AP incubated at 1:15000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis using 10736-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10736-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10736-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lung using 10736-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 10736-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 10736-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10736-1-AP

  IP Result of anti-VCP (IP:10736-1-AP, 4ug; Detection:10736-1-AP 1:1000) with HeLa cells lysate 1505ug.

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  IP experiment of mouse brain using 10736-1-AP

  IP result of anti-VCP (IP:10736-1-AP, 4ug; Detection:10736-1-AP 1:300) with mouse brain tissue lysate 2400 ug.

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  IHC staining of human gliomas using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10736-1-AP (VCP Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human gliomas using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10736-1-AP (VCP Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human gliomas using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules NIH/3T3, cellules SH-SY5Y, cellules U2OS, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu de thymus de souris, tissu pulmonaire de rat, tissu pulmonaire de souris
• Résultats positifs en IP: cellules HeLa, tissu cérébral de souris
• Résultats positifs en IHC: tissu de gliome humain, tissu de cancer du côlon humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 33 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

10736-1-AP cible VCP dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VCP Protéine recombinante Ag1002
• Nom complet: valosin-containing protein
• Masse moléculaire calculée: 89 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC007562
• Symbole du gène: VCP
• Identification du gène (NCBI): 7415
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).

Publications

Species	Application	Title
mouse	WB	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo
human	WB	J Clin Invest; Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders; Authors - Huilun Helen Wang
human	WB	Sci Adv; Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.; Authors - Yuan Wang
human	WB	Nat Commun; Analysis of Zika virus capsid-Aedes aegypti mosquito interactome reveals pro-viral host factors critical for establishing infection.; Authors - Rommel J Gestuveo ; KD Validated
human	WB	Cell Chem Biol; Targeting non-canonical pathways as a strategy to modulate the sodium iodide symporter.; Authors - Martin L Read ; KD Validated
human	WB	Hum Mol Genet; Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.; Authors - Zhouteng Tao