Anticorps Polyclonal de lapin anti-VDAC1/Porin

• Phare
• Validé par KD/KO

VDAC1/Porin Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (7)

Applications

WB, IHC, IF-P, IF-Fro, FC (Intra), CoIP, ELISA, PLA

Conjugaison

Non conjugué

Publications(203)

Avis (1)

N° de cat : 55259-1-AP

Synonymes

Porin, VDAC1, hVDAC1, Plasmalemmal porin, PORIN 31 HL

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• Galerie de données de validation
• Proteintech vs. Other Vendors

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  WB analysis of HeLa cells using 55259-1-AP

  WB result of VDAC1 antibody (55259-1-AP, 1:2000) with si-Control and si-VDAC1 transfected HeLa cells.

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  WB analysis using 55259-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 55259-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 55259-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 55259-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55259-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55259-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 55259-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55259-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 55259-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 55259-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 55259-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 55259-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 55259-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 55259-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of human liver cancer using 55259-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using VDAC1/Porin antibody (55259-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse liver using 55259-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse liver tissue using VDAC1/Porin antibody (55259-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 55259-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human VDAC1/Porin (55259-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, cellules A431, cellules HEK-293, cellules HeLa, cellules HepG2, cellules NIH/3T3, cellules ROS1728, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de rat, tissu cérébral de souris, tissu de muscle squelettique humain, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du foie humain,
• Résultats positifs en IF-Fro: tissu hépatique de souris,
• Résultats positifs en FC (Intra): cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 184 publications below
• IHC: See 7 publications below
• IF: See 15 publications below
• CoIP: See 5 publications below

Informations sur le produit

55259-1-AP cible VDAC1/Porin dans les applications de WB, IHC, IF-P, IF-Fro, FC (Intra), CoIP, ELISA, PLA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, canin, Humain, porc, poulet, singe, souris, Hamster, duck
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: voltage-dependent anion channel 1
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: NM_003374
• Symbole du gène: Porin
• Identification du gène (NCBI): 7416
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This antibody is specific to VDAC1.

Publications

Species	Application	Title
human	WB	Cell; IRGQ-mediated autophagy in MHC class I quality control promotes tumor immune evasion; Authors - Lina Herhaus
human	WB	Nat Commun; MYG1 drives glycolysis and colorectal cancer development through nuclear-mitochondrial collaboration; Authors - Jianxiong Chen
human	WB	Mol Cell; Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology; Authors - Gayathri Muthukumar
		Mol Cell; Hexokinase 1 cellular localization regulates the metabolic fate of glucose.; Authors - Adam De Jesus
monkey	WB	Nat Commun; An ESCRT-dependent step in fatty acid transfer from lipid droplets to mitochondria through VPS13D-TSG101 interactions.; Authors - Jingru Wang
human	WB	Nucleic Acids Res; The human tRNA taurine modification enzyme GTPBP3 is an active GTPase linked to mitochondrial diseases.; Authors - Gui-Xin Peng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ying (Verified Customer) (04-22-2021)

good antibody. works very well with mouse cell lines.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000

VDAC1/Porin Antibody Western Blot validation (1:2000 dilution) in (Cat no:55259-1-AP)

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