Anticorps Recombinant de lapin anti-VDAC1/Porin

VDAC1/Porin Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

2F4

Avis (2)

N° de cat : 81538-1-RR

Synonymes

Porin, VDAC1, Porin 31HL, PORIN 31 HL, Plasmalemmal porin

---

Galerie de données de validation

• 

  WB analysis using 81538-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81538-1-RR (VDAC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 81538-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81538-1-RR (VDAC1/Porin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 81538-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81538-1-RR (VDAC1/Porin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver using 81538-1-RR

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using VDAC1/Porin antibody (81538-1-RR, Clone: 2F4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 81538-1-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug VDAC1/Porin Recombinant antibody (81538-1-RR, Clone:2F4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules MDA-MB-231, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NIH/3T3
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu hépatique humain,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

81538-1-RR cible VDAC1/Porin dans les applications de WB, IHC, IF-P, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: voltage-dependent anion channel 1
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: NM_003374
• Symbole du gène: Porin
• Identification du gène (NCBI): 7416
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This antibody is specific to VDAC1.

Publications

Species	Application	Title
mouse	WB	J Nutr Biochem; Iron-frataxin involved in the protective effect of quercetin against alcohol-induced liver mitochondrial dysfunction; Authors - Jingjing Liu
rat	WB	Int J Nanomedicine; Mitochondria-Rich Microvesicles Alleviate CNI ED by Transferring Mitochondria and Suppressing Local Ferroptosis; Authors - Zhenkang Liang
rat	WB,IP	Phytother Res; Pubescenoside D Ameliorates Myocardial Ischemia-Reperfusion Injury via Preventing the Dissociation of HK2 and Promoting Mitophagy by Targeting GSK-3β; Authors - Juanlan Xiao
	WB	Plant Physiol; The wheat stripe rust effector PstEXLX1 inhibits formate dehydrogenase activity to suppress immunity in wheat; Authors - Jingwei Lu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Vignesh (Verified Customer) (09-03-2025)

It's an excellent product with reduced or no nonspecific bands and clear band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: 231 cell line

FH

Monifa (Verified Customer) (08-31-2025)

The VDAC antibody worked well as a control for mitochondrial marker in organelle isolation. I used 1% BSA overnight in 4C. I visualized with HRP secondaries and signal is high. Will try higher dilutions like 1:5000 as recommended and for shorter time at room temperature to speed up the completion of my blots.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: HEK293

{{ptg:RelatedPrimaryAntibodies}}