Anticorps Polyclonal de lapin anti-Vitamin D binding protein

Vitamin D binding protein Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 16922-1-AP

Synonymes

GC, VDBP,GC, Gc protein-derived macrophage activating factor, Gc globulin, DBP-maf

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Galerie de données de validation

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  WB analysis of human plasma using 16922-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 16922-1-AP (vitamin D binding protein antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human blood using 16922-1-AP

  human blood tissue were subjected to SDS PAGE followed by western blot with 16922-1-AP (vitamin D binding protein antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat eye using 16922-1-AP

  rat eye tissue were subjected to SDS PAGE followed by western blot with 16922-1-AP (Vitamin D binding protein antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16922-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16922-1-AP (vitamin D binding protein antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 16922-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 16922-1-AP (vitamin D binding protein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16922-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16922-1-AP (vitamin D binding protein antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 16922-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 16922-1-AP (vitamin D binding protein antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16922-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16922-1-AP (Vitamin D binding protein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16922-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16922-1-AP (Vitamin D binding protein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 16922-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16922-1-AP (Vitamin D binding protein antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu sanguin humain, cellules PC-3, plasma humain, sang humain, Sanguin humain, tissu oculaire de rat, tissu oculaire de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu hépatique humain, human normal colon; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

16922-1-AP cible Vitamin D binding protein dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Vitamin D binding protein Protéine recombinante Ag10286
• Nom complet: group-specific component (vitamin D binding protein)
• Masse moléculaire calculée: 474 aa, 53 kDa
• Poids moléculaire observé: 52-58 kDa
• Numéro d’acquisition GenBank: BC057228
• Symbole du gène: Vitamin D binding protein
• Identification du gène (NCBI): 2638
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vitamin D binding protein is a sparsely glycosylated serum protein responsible for highly specific binding and tissue-specific delivery of vitamin D and its metabolites. In addition, it is also an actin scavenger, and is the precursor to the immunomodulatory protein, Gc-MAF. Vitamin D binding protein has been proposed to have significant roles in C5a chemotaxis, osteoclast development and possibly in macrophage activation/recruitment.

Publications

Species	Application	Title
mouse	WB	Mol Ther Methods Clin Dev; Lentiviral vector mediated gene therapy for type I Dent disease ameliorates Dent disease-like phenotypes for three months in ClC-5 null mice; Authors - Manish Kumar Yadav
rat	WB	Sci Rep; PMID: 31792309; Authors - Nela Pavlíková
		Proteomics; Screening for potential serum biomarkers in rat mesangial proliferative nephritis.; Authors - Yang Lu
rat	WB,IHC	Mol Med Rep; Proteomics of acute heart failure in a rat post-myocardial infarction model.; Authors - Yichen Guo
mouse	WB	Pharmaceutics; Evaluation of In Vitro and In Vivo Antiviral Activities of Vitamin D for SARS-CoV-2 and Variants; Authors - Chee-Keng Mok
mouse	IHC	Int Immunopharmacol; Vitamin D attenuates DNCB-induced atopic dermatitis-like skin lesions by inhibiting immune response and restoring skin barrier function; Authors - Renwei Lu

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