Anticorps Recombinant de lapin anti-VEGFR1/Flt-1

VEGFR1/Flt-1 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

242311D5

N° de cat : 98418-2-RR

Synonymes

Flt1, FLT-1, VEGFR-1, VGFR1

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Galerie de données de validation

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  FC experiment of Transfected HEK-293T using 98418-2-RR

  1x10^6 HEK-293T cells or VEGFR1/Flt-1 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat VEGFR1/Flt-1 Rabbit RecAb (98418-2-RR, Clone:242311D5) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected HEK-293T using 98418-2-RR

  1x10^6 HEK-293T cells or VEGFR1/Flt-1 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat VEGFR1/Flt-1 Rabbit RecAb (98418-2-RR, Clone: 242311D5) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98418-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98418-2-RR against Rat VEGFR1/Flt-1 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en FC (Intra): Transfected HEK-293T cells,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98418-2-RR cible VEGFR1/Flt-1 dans les applications de FC (Intra) et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: VEGFR1/Flt-1 Protéine recombinante Eg1167
• Nom complet: fms-related tyrosine kinase 1
• Symbole du gène: Flt1
• Identification du gène (NCBI): 54251
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Vascular endothelial growth factor receptor-1 (VEGFR-1, FLT-1) is a receptor tyrosine kinase belonging to the VEGFR family. VEGF is a key regulator of physiological angiogenesis and has also been implicated in pathological angiogenesis associated with tumors, intraocular neovascular disorders, and other conditions. The biological effects of VEGF are mediated by VEGFR-1 and VEGFR-2. Both the two receptors have seven immunoglobulin-like repeats in the extracellular domain, a single transmembrane region and a tyrosine kinase domain. VEGFR-1 binds VEGFA, PIGF, and VEGFB, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion.

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